HCN4 Polyclonal antibody

HCN4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 55224-1-AP

Synonyms

HCN4

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Validation Data Gallery

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  WB analysis using 55224-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55224-1-AP (HCN4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below

Product Information

55224-1-AP targets HCN4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: hyperpolarization activated cyclic nucleotide-gated potassium channel 4
• Calculated Molecular Weight: 129 kDa
• Observed Molecular Weight: 140-160 kDa
• GenBank Accession Number: NM_005477
• Gene Symbol: HCN4
• Gene ID (NCBI): 10021
• RRID: AB_11182714
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HCN4 belongs to the potassium channel HCN family. It hyperpolarization-activated ion channel with very slow activation and inactivation exhibiting weak selectivity for potassium over sodium ions. HCN4 may contribute to the native pacemaker currents in heart (If) and in neurons (Ih). It is activated by cAMP. HCN4 may mediate responses to sour stimuli. (PMID:10228147). Defects in HCN4 are a cause of sick sinus syndrome type 2 (SSS2). Defects in HCN4 are the cause of Brugada syndrome type 8 (BRS8). This antibody is specific to HCN4.

Publications

Species	Application	Title
hamster	WB	Proc Natl Acad Sci U S A; A gain-of-function HCN4 mutant in the HCN domain is responsible for inappropriate sinus tachycardia in a Spanish family; Authors - Anabel Cámara-Checa
human	WB	iScience; Engineering of thick human functional myocardium via static stretching and electrical stimulation.; Authors - Danielle Pretorius
rat	WB	Bull Exp Biol Med; Glucocorticoid Receptor/HCN4 Channels Interaction in Spinal Dorsal Horn Participates in the Regulation of Neuropathic Pain after Peripheral Nerve Injury in Rats; Authors - L Zhengshuai
rat	WB	Mol Med Rep; G9a inhibition promotes the formation of pacemaker-like cells by reducing the enrichment of H3K9me2 in the HCN4 promoter region; Authors - Pei Xu
rat	WB	PLoS One; DNA methylation cooperates with H3K9me2 at HCN4 promoter to regulate the differentiation of bone marrow mesenchymal stem cells into pacemaker-like cells; Authors - XiaoLin Sun