HCN4 Polyclonal antibody

HCN4 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 55224-1-AP



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 5 publications below

Product Information

55224-1-AP targets HCN4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name hyperpolarization activated cyclic nucleotide-gated potassium channel 4
Calculated Molecular Weight 129 kDa
Observed Molecular Weight140-160 kDa
GenBank Accession NumberNM_005477
Gene Symbol HCN4
Gene ID (NCBI) 10021
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HCN4 belongs to the potassium channel HCN family. It hyperpolarization-activated ion channel with very slow activation and inactivation exhibiting weak selectivity for potassium over sodium ions. HCN4 may contribute to the native pacemaker currents in heart (If) and in neurons (Ih). It is activated by cAMP. HCN4 may mediate responses to sour stimuli. (PMID:10228147). Defects in HCN4 are a cause of sick sinus syndrome type 2 (SSS2). Defects in HCN4 are the cause of Brugada syndrome type 8 (BRS8). This antibody is specific to HCN4.


Product Specific Protocols
WB protocol for HCN4 antibody 55224-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Proc Natl Acad Sci U S A

A gain-of-function HCN4 mutant in the HCN domain is responsible for inappropriate sinus tachycardia in a Spanish family

Authors - Anabel Cámara-Checa


Engineering of thick human functional myocardium via static stretching and electrical stimulation.

Authors - Danielle Pretorius

Bull Exp Biol Med

Glucocorticoid Receptor/HCN4 Channels Interaction in Spinal Dorsal Horn Participates in the Regulation of Neuropathic Pain after Peripheral Nerve Injury in Rats

Authors - L Zhengshuai

Mol Med Rep

G9a inhibition promotes the formation of pacemaker-like cells by reducing the enrichment of H3K9me2 in the HCN4 promoter region

Authors - Pei Xu

PLoS One

DNA methylation cooperates with H3K9me2 at HCN4 promoter to regulate the differentiation of bone marrow mesenchymal stem cells into pacemaker-like cells

Authors - XiaoLin Sun