HCN1 Polyclonal antibody

HCN1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 55222-1-AP

Synonyms

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, HAC 2, Brain cyclic nucleotide-gated channel 1, BCNG-1

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Validation Data Gallery

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  WB analysis of mouse brain using 55222-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55222-1-AP (HCN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 55222-1-AP

  IP result of anti-HCN1 (IP:55222-1-AP, 4ug; Detection:55222-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human heart using 55222-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55222-1-AP (HCN1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cerebellum using 55222-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 55222-1-AP (HCN1 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human heart tissue, human cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

55222-1-AP targets HCN1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: hyperpolarization activated cyclic nucleotide-gated potassium channel 1
• Calculated Molecular Weight: 99 kDa
• Observed Molecular Weight: 99-120 kDa
• GenBank Accession Number: NM_021072
• Gene Symbol: HCN1
• Gene ID (NCBI): 348980
• RRID: AB_11182929
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HCN1, also named as BCNG1, belongs to the potassium channel HCN family. It hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. HCN1 contributes to the native pacemaker currents in heart (If) and in neurons (Ih). It is activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. HCN1 may mediate responses to sour stimuli.

Publications

Species	Application	Title
mouse	IF	Neuron; Cellular and circuit architecture of the lateral septum for reward processing; Authors - Gaowei Chen
mouse	WB	J Affect Disord; Prenatal alcohol exposure enhanced alcohol preference and susceptibility to PTSD in a sex-dependent manner through the synaptic HCN1 channel; Authors - Hui Yao
human	WB	iScience; Using brain cell-type-specific protein interactomes to interpret neurodevelopmental genetic signals in schizophrenia; Authors - Yu-Han H Hsu
mouse	WB	Cell Rep; Optogenetic Activation of Striatopallidal Neurons Reveals Altered HCN Gating in DYT1 Dystonia.; Authors - Giuseppe Sciamanna
mouse	WB	Neurosci Bull; Thalamocortical Circuit Controls Neuropathic Pain via Up-regulation of HCN2 in the Ventral Posterolateral Thalamus; Authors - Yi Yan
mouse	WB,IHC	CNS Neurosci Ther; HCN channels in the lateral habenula regulate pain and comorbid depressive-like behaviors in mice; Authors - Xue-Zhong Cao