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GSDMD Polyclonal antibody
GSDMD Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Conjugate
Unconjugated
254
Cat no : 20770-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, NIH/3T3 cells, Jurkat cells, C6 cells, rat liver tissue, rat brain tissue |
Positive IP detected in | THP-1 cells |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 6 publications below |
WB | See 202 publications below |
IHC | See 51 publications below |
IF | See 68 publications below |
FC | See 1 publications below |
Product Information
20770-1-AP targets GSDMD in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig, bovine, hamster |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | GSDMD fusion protein Ag14757 |
Full Name | gasdermin D |
Calculated Molecular Weight | 484 aa, 53 kDa |
Observed Molecular Weight | 50-53 kDa |
GenBank Accession Number | BC008904 |
Gene Symbol | GSDMD |
Gene ID (NCBI) | 79792 |
RRID | AB_10696319 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GSDMD is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. GSDMD has been suggested to act as a tumor suppressor. Recently, GSDMD is identified as a new component of inflammasomes, and it is an executor of pyroptosis and required for interleukin-1β secretion. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death.
Protocols
Product Specific Protocols | |
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WB protocol for GSDMD antibody 20770-1-AP | Download protocol |
IF protocol for GSDMD antibody 20770-1-AP | Download protocol |
IP protocol for GSDMD antibody 20770-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nature Inflammasome-activated gasdermin D causes pyroptosis by forming membrane pores.
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ACS Nano Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration. | ||
Acta Pharm Sin B Miltirone induces cell death in hepatocellular carcinoma cell through GSDME-dependent pyroptosis. | ||
Acta Pharm Sin B A high-throughput Gaussia luciferase reporter assay for screening potential gasdermin E activators against pancreatic cancer | ||
Cell Res Gasdermin D is an executor of pyroptosis and required for interleukin-1β secretion. | ||
J Exp Med Protein kinase D at the Golgi controls NLRP3 inflammasome activation.
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Isha (Verified Customer) (03-02-2021) | Bands were so clear. Could not refrain myself from writing this review.
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