GRPEL1 Polyclonal antibody

GRPEL1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12720-1-AP

Synonyms

GREPEL1, GRPEL1, HMGE, Mt GrpE#1

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Validation Data Gallery

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  WB analysis using 12720-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12720-1-AP (GRPEL1 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12720-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12720-1-AP (GRPEL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 12720-1-AP

  IP Result of anti-GRPEL1 (IP:12720-1-AP, 4ug; Detection:12720-1-AP 1:600) with MCF-7 cells lysate 1280ug.

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  IHC staining of human colon cancer using 12720-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12720-1-AP (GRPEL1 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HepG2 using 12720-1-AP

  Immunofluorescent analysis of HepG2 cells, using GRPEL1 antibody 12720-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: MCF-7 cells, HEK-293T cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 3 publications below

Product Information

12720-1-AP targets GRPEL1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GRPEL1 fusion protein Ag3415
• Full Name: GrpE-like 1, mitochondrial (E. coli)
• Calculated molecular weight: 217 aa, 24 kDa
• Observed molecular weight: 24 kDa
• GenBank accession number: BC024242
• Gene symbol: GRPEL1
• Gene ID (NCBI): 80273
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding.; Authors - Chun-Shik Shin
human	WB	Nat Commun; Protein import motor complex reacts to mitochondrial misfolding by reducing protein import and activating mitophagy; Authors - Jonas Benjamin Michaelis
human	WB	EBioMedicine; The LIV-1-GRPEL1 axis adjusts cell fate during anti-mitotic agent-damaged mitosis.; Authors - Pingbo Chen