GRP78,BIP Recombinant antibody

• Featured Product
• KD/KO Validated

GRP78,BIP Uni-rAb^TM Recombinant Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

5F17

Cat no : 80849-1-RR

Synonyms

BIP, GRP78, GRP78/BIP, HSPA5, Endoplasmic reticulum chaperone BiP

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Validation Data Gallery

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  WB analysis of HepG2 using 80849-1-RR

  WB result of GRP78,BIP antibody (80849-1-RR; 1:16000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GRP78,BIP transfected HepG2 cells.

• 

  WB analysis using 80849-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80849-1-RR (GRP78,BIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 80849-1-RR

  IP result of anti-GRP78,BIP (IP:80849-1-RR, 4ug; Detection:80849-1-RR 1:3000) with HeLa cells lysate 1280 ug.

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  IHC staining of human colon cancer using 80849-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80849-1-RR (GRP78,BIP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 80849-1-RR

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80849-1-RR (GRP78,BIP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 80849-1-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80849-1-RR (GRP78,BIP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 80849-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80849-1-RR against Human GRP78/BIP were performed. The affinity constant is 4.08 nM.

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, RAW264.7 cells, HSC-T6 cells, pig liver tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue, human pancreas cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

80849-1-RR targets GRP78,BIP in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GRP78,BIP fusion protein Ag2188
• Full Name: heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 70,78 kDa
• GenBank Accession Number: BC020235
• Gene Symbol: GRP78/BIP
• Gene ID (NCBI): 3309
• RRID: AB_2918916
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
pig	IF	J Agric Food Chem; Pterostilbene Prevents Tunicamycin-Induced Intestinal Barrier Damage by Targeting Endoplasmic Reticulum Stress, Oxidative Stress, Autophagy, and Gut Microbiota; Authors - Yanan Chen