mGluR2 Polyclonal antibody

mGluR2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 19956-1-AP

Synonyms

GLUR2, GPRC1B, GRM2, mGlu2, mGluR2

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Validation Data Gallery

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  WB analysis of human brain using 19956-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19956-1-AP (mGluR2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19956-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19956-1-AP (mGluR2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 19956-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 19956-1-AP (mGluR2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 19956-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19956-1-AP (mGluR2 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse brain using 19956-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19956-1-AP (mGluR2 antibody at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: human brain tissue, HeLa cells, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below

Product Information

19956-1-AP targets mGluR2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: glutamate receptor, metabotropic 2
• Calculated Molecular Weight: 96 kDa
• Observed Molecular Weight: 96 kDa, 120 kDa
• GenBank Accession Number: NM_000839
• Gene Symbol: mGluR2
• Gene ID (NCBI): 2912
• RRID: AB_10638447
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRM2, also named as GPRC1B and MGLUR2, belongs to the G-protein coupled receptor 3 family. GRM2 is a receptor for glutamate. The activity of GRM2 is mediated by a G-protein that inhibits adenylate cyclase activity. It may mediate suppression of neurotransmission or may be involved in synaptogenesis or synaptic stabilization.

Publications

Species	Application	Title
human	WB	Mol Cancer Res; Long-range epigenetic silencing associates with deregulation of Ikaros targets in colorectal cancer cells.; Authors - Javierre Biola M BM
rat	WB,IHC	Auton Neurosci; Expression of group II metabotropic glutamate receptors in rat superior cervical ganglion; Authors - Xixi Wei
rat	WB,IHC	Neurochem Int; Red nucleus mGluR2 but not mGluR3 mediates inhibitory effect in the development of SNI-induced neuropathological pain by suppressing the expressions of TNF-α and IL-1β; Authors - Wen-Tao Wang