GRID1 Polyclonal antibody

GRID1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13040-1-AP

Synonyms

KIAA1220, Glutamate receptor ionotropic, delta-1, GluR delta-1 subunit, GluR delta 1 subunit, GluD1

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Validation Data Gallery

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  WB analysis of human brain using 13040-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13040-1-AP (GRID1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 13040-1-AP

  IP result of anti-GRID1 (IP:13040-1-AP, 4ug; Detection:13040-1-AP 1:300) with mouse brain tissue lysate 5600ug.

• 

  IHC staining of human gliomas using 13040-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13040-1-AP (GRID1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 13040-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13040-1-AP (GRID1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 13040-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13040-1-AP (GRID1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 13040-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13040-1-AP (GRID1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13040-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13040-1-AP (GRID1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13040-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13040-1-AP (GRID1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue, human brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

13040-1-AP targets GRID1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GRID1 fusion protein Ag3694
• Full Name: glutamate receptor, ionotropic, delta 1
• Calculated Molecular Weight: 1009 aa, 112 kDa
• Observed Molecular Weight: 100-112 kDa
• GenBank Accession Number: BC039263
• Gene Symbol: GRID1
• Gene ID (NCBI): 2894
• RRID: AB_10638439
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Anesthesiology; Single-nucleus Atlas of Sevoflurane-induced Hippocampal Cell Type- and Sex-specific Effects during Development in Mice; Authors - Shao-Yong Song