GRAP Polyclonal antibody

GRAP Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14505-1-AP

Synonyms

GRAP, GRB2 related adapter protein, GRB2 related adaptor protein

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Validation Data Gallery

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  WB analysis of U-937 using 14505-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 14505-1-AP (GRAP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 14505-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 14505-1-AP (GRAP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 14505-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 14505-1-AP (GRAP antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

14505-1-AP targets GRAP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GRAP fusion protein Ag5962
• Full Name: GRB2-related adaptor protein
• Calculated Molecular Weight: 25 kDa
• GenBank Accession Number: BC063035
• Gene Symbol: GRAP
• Gene ID (NCBI): 10750
• RRID: AB_2112704
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse,human	WB	Life Sci; Grb2-related adaptor protein GRAP is a novel regulator of liver fibrosis; Authors - Xiaoyan Wu
mouse	WB	Life Sci; Ablation of Brg1 in fibroblast/myofibroblast lineages attenuates renal fibrosis in mice with diabetic nephropathy; Authors - Xiaoyan Wu