GPI Polyclonal antibody

• Featured Product
• KD/KO Validated

GPI Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

20

Reviews (2)

Cat no : 15171-1-AP

Synonyms

AMF, Autocrine motility factor, g6pi, Glucose 6 phosphate isomerase, glucose phosphate isomerase, GNPI, GPI, Neuroleukin, NLK, PGI, PHI, Phosphoglucose isomerase, Phosphohexose isomerase, SA 36, Sperm antigen 36

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Validation Data Gallery

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  WB analysis using 15171-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15171-1-AP (GPI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15171-1-AP

  WB result of GPI antibody (15171-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPI transfected HeLa cells.

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  WB analysis of HeLa using 15171-1-AP

  WB result of GPI antibody (15171-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPI transfected HeLa cells.

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  WB analysis of PC-3 using 15171-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15171-1-AP (GPI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15171-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15171-1-AP (GPI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 15171-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15171-1-AP (GPI Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 15171-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15171-1-AP (GPI Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 15171-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 15171-1-AP (GPI antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, PC-3 cells, PC3 cells, U251 cells, U87-MG cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 18 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

15171-1-AP targets GPI in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPI fusion protein Ag7423
• Full Name: glucose phosphate isomerase
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 55-64 kDa
• GenBank Accession Number: BC004982
• Gene Symbol: GPI
• Gene ID (NCBI): 2821
• RRID: AB_2263537
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPI(Glucose-6-phosphate isomerase), which is also named as autocrine motility factor (AMF), phosphoglucose isomerase(PGI), Neuroleukinis(NLK), phosphohexose isomerase(PHI) or sperm antigen 36(SA-36), is a housekeeping cytosolic enzyme that plays a key role in both glycolysis and gluconeogenesis pathways. It is also a multifunctional protein that displays cytokine properties, eliciting mitogenic, motogenic, and differentiation activities, and has been implicated in tumor progression and metastasis (PMID:12783864, 19603112). This protein can exsit as a homodimer in the catalytically active form and a monomer in the secreted form (PMID:11371164). It has 2 isoforms produced by alternative splicing with the calculated molecular mass of 63-64kDa, and an apparent molecular mass of 55 and 64 kDa under non-reducing and reducing conditions, respectively(PMID: 19603112, 11004567).

Publications

Species	Application	Title
human	WB	Cell Res; Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P; Authors - Yuan-Li Ai
mouse	IF	Nat Neurosci; A glycolytic shift in Schwann cells supports injured axons.; Authors - Elisabetta Babetto
human	WB	Cell Res; cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.; Authors - Linchong Sun
mouse	WB	EMBO J; Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.; Authors - Lihua Wang
human	WB	EMBO Rep; CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.; Authors - Xiuying Zhong
human	WB	EMBO Rep; OMA1 reprograms metabolism under hypoxia to promote colorectal cancer development.; Authors - Zhida Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kristian (Verified Customer) (07-26-2021)

GPI is shown in green. Nuclei are shown in blue.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: RCC4

GPI Antibody Immunofluorescence validation (1:200 dilution) in RCC4 (Cat no:15171-1-AP)

FH

Daniel (Verified Customer) (02-06-2020)

Denatured lysate was separated on a 4-20% PAGE

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse kidney

GPI Antibody Western Blot, validation (1:2000 dilution) in mouse kidney (Cat no:15171-1-AP)