Validation Data Gallery
|Positive WB detected in||HeLa cells, PC-3 cells, PC3 cells, U251 cells, U87-MG cells|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||PC-3 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15171-1-AP targets GPI in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GPI fusion protein Ag7423|
|Full Name||glucose phosphate isomerase|
|Calculated molecular weight||63 kDa|
|Observed molecular weight||55-64 kDa|
|GenBank accession number||BC004982|
|Gene ID (NCBI)||2821|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
GPI(Glucose-6-phosphate isomerase), which is also named as autocrine motility factor (AMF), phosphoglucose isomerase(PGI), Neuroleukinis(NLK), phosphohexose isomerase(PHI) or sperm antigen 36(SA-36), is a housekeeping cytosolic enzyme that plays a key role in both glycolysis and gluconeogenesis pathways. It is also a multifunctional protein that displays cytokine properties, eliciting mitogenic, motogenic, and differentiation activities, and has been implicated in tumor progression and metastasis (PMID:12783864, 19603112). This protein can exsit as a homodimer in the catalytically active form and a monomer in the secreted form (PMID:11371164). It has 2 isoforms produced by alternative splicing with the calculated molecular mass of 63-64kDa, and an apparent molecular mass of 55 and 64 kDa under non-reducing and reducing conditions, respectively(PMID: 19603112, 11004567).
Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P
A glycolytic shift in Schwann cells supports injured axons.
cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.
Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.
CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.
OMA1 reprograms metabolism under hypoxia to promote colorectal cancer development.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kristian (Verified Customer) (07-26-2021)
GPI is shown in green. Nuclei are shown in blue.
Daniel (Verified Customer) (02-06-2020)
Denatured lysate was separated on a 4-20% PAGE