GOLPH3 Polyclonal antibody

• Featured Product
• KD/KO Validated

GOLPH3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

24

Reviews (1)

Cat no : 19112-1-AP

Synonyms

MIDAS, GPP34, GOPP1, GOLPH 3, Golgi phosphoprotein 3

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Validation Data Gallery

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  WB analysis using 19112-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19112-1-AP (GOLPH3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 19112-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 19112-1-AP (GOLPH3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 19112-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19112-1-AP (GOLPH3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 19112-1-AP

  IP result of anti-GOLPH3 (IP:19112-1-AP, 3ug; Detection:19112-1-AP 1:1500) with mouse testis tissue lysate 4000ug.

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  IHC staining of human colon cancer using 19112-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 19112-1-AP (GOLPH3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 19112-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 19112-1-AP (GOLPH3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 19112-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 19112-1-AP (GOLPH3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 19112-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 19112-1-AP (GOLPH3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 19112-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19112-1-AP (GOLPH3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 19112-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19112-1-AP (GOLPH3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 19112-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 19112-1-AP (GOLPH3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 19112-1-AP

  Immunofluorescent analysis of HepG2 cells using 19112-1-AP (GOLPH3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: HeLa cells, mouse lung tissue, rat testis tissue, mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human colon cancer tissue, human placenta tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 9 publications below
• WB: See 17 publications below
• IHC: See 10 publications below
• IF: See 5 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

19112-1-AP targets GOLPH3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GOLPH3 fusion protein Ag5443
• Full Name: golgi phosphoprotein 3 (coat-protein)
• Calculated Molecular Weight: 298 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC033725
• Gene Symbol: GOLPH3
• Gene ID (NCBI): 64083
• RRID: AB_2113342
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GOLPH3 (also called GPP34, GMx33, MIDAS, or yeast Vps74p) is a 34-kDa Golgi-associated protein conserved from yeast to human. GOLPH3 binds to PtdIns(4)P-rich trans-Golgi membranes and MYO18A conveying a tensile force required for efficient tubule and vesicle formation (PMID: 19837035). GOLPH3 has been recently demonstrated as a novel oncoprotein amplified in various types of human malignancies, including melanoma, breast, non-small cell lung cancer, gliomas and connective tissue tumors (PMID:19553991; 23006319; 21499727; 22745132). Enhanced activation of mTOR signalling represents a molecular basis for the oncogenic activity of GOLPH3 (PMID: 19553991).

Publications

Species	Application	Title
human,mouse	WB,IHC,IF	Theranostics; miR34a/GOLPH3 Axis abrogates Urothelial Bladder Cancer Chemoresistance via Reduced Cancer Stemness.; Authors - Qing Zhang ; KD Validated
human	WB	Cancer Res; A Novel Micropeptide Encoded by Y-Linked LINC00278 Links Cigarette Smoking and AR Signaling in Male Esophageal Squamous Cell Carcinoma.; Authors - Siqi Wu
human	WB	Cell Death Dis; GOLPH3/CKAP4 promotes metastasis and tumorigenicity by enhancing the secretion of exosomal WNT3A in non-small-cell lung cancer; Authors - Jun-Wei Song
human	IHC	Front Oncol; FAP-a and GOLPH3 Are Hallmarks of DCIS Progression to Invasive Breast Cancer.; Authors - Li-Na Yu
		Br J Cancer; GOLPH3 is a novel marker of poor prognosis and a potential therapeutic target in human renal cell carcinoma.; Authors - Y Xue
human	WB	J Exp Clin Cancer Res; STK25-induced inhibition of aerobic glycolysis via GOLPH3-mTOR pathway suppresses cell proliferation in colorectal cancer.; Authors - Fan Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (04-17-2023)

fairly good for IF, but not as clear as the images in its booklet

• Applications: Immunofluorescence

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