GP73/GOLPH2 Polyclonal antibody

• Featured Product
• KD/KO Validated

GP73/GOLPH2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 15126-1-AP

Synonyms

GOLM1, GOLPH2, GP73, GOLM 1, golgi protein 73kD

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Validation Data Gallery

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  WB analysis using 15126-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15126-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15126-1-AP

  IP result of anti-GP73/GOLPH2 (IP:15126-1-AP, 4ug; Detection:15126-1-AP 1:1000) with HeLa cells lysate 2800ug.

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  IHC staining of human stomach using 15126-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15126-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15126-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 15126-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15126-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15126-1-AP (GP73/GOLPH2 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The cytoskeleton and nuclei were stained with CL594-phalloidin and DAPI, respectively.

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  IF Staining of HeLa using 15126-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GP73/GOLPH2 antibody (15126-1-AP) at dilution of 1:1800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 15126-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GP73/GOLPH2 antibody (15126-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 15126-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15126-1-AP (GP73/GOLPH2 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: staning with CoraLite555-Phalloidin.

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Tested Applications

• Positive WB detected in: LNCaP cells, HuH-7 cells, MDA-MB-231 cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human stomach cancer tissue, human liver cancer tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 6 publications below

Product Information

15126-1-AP targets GP73/GOLPH2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GP73/GOLPH2 fusion protein Ag7207
• Full Name: golgi membrane protein 1
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC001740
• Gene Symbol: GP73
• Gene ID (NCBI): 51280
• RRID: AB_2113330
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 70-75 kDa in western blotting due to phosphorylation.

Publications

Species	Application	Title
human	WB	PLoS Pathog; GP73 represses host innate immune response to promote virus replication by facilitating MAVS and TRAF6 degradation.; Authors - Xuewu Zhang ; KD Validated
human	WB	J Cell Sci; Rapid affinity purification of intracellular organelles using a twin strep tag.; Authors - Jian Xiong
human	IF	Chemistry; Investigation of Glycosylphosphatidylinositol (GPI)-Plasma Membrane Interaction in Live Cells and the Influence of GPI Glycan Structure on the Interaction; Authors - Sayan Kundu
mouse	WB,IF	Int J Med Sci; Causal Association of Golgi Protein 73 With Coronary Artery Disease: Evidence from Proteomics and Mendelian Randomization; Authors - Yi-Fen Lin
human	WB,IHC,IF	J Cancer; GP73 level determines chemotherapeutic resistance in human hepatocellular carcinoma cells.; Authors - Jia-Zhou Ye ; KD Validated
		Curr Alzheimer Res; RCAN1 Increases Aβ Generation by Promoting N-Glycosylation Via Oligosaccharyl Transferase.; Authors - T Wang