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  • KD/KO Validated

GP73/GOLPH2 Polyclonal antibody

GP73/GOLPH2 Polyclonal Antibody for WB, IP, IF, FC, ELISA

Host / Isotype

Rabbit / IgG


human and More (1)





Cat no : 15126-1-AP


golgi membrane protein 1, Golgi membrane protein GP73, Golgi phosphoprotein 2, golgi protein 73kD, GOLM1, GOLPH2, GP73, GP73/GOLPH2

Tested Applications

Positive WB detected inLNCaP cells, HuH-7 cells, MDA-MB-231 cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15126-1-AP targets GP73/GOLPH2 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GP73/GOLPH2 fusion protein Ag7207
Full Name golgi membrane protein 1
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC001740
Gene Symbol GOLM1
Gene ID (NCBI) 51280
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 70-75 kDa in western blotting due to phosphorylation.


Product Specific Protocols
WB protocol for GP73/GOLPH2 antibody 15126-1-APDownload protocol
IHC protocol for GP73/GOLPH2 antibody 15126-1-APDownload protocol
IF protocol for GP73/GOLPH2 antibody 15126-1-APDownload protocol
IP protocol for GP73/GOLPH2 antibody 15126-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



PLoS Pathog

GP73 represses host innate immune response to promote virus replication by facilitating MAVS and TRAF6 degradation.

Authors - Xuewu Zhang
  • KD Validated

J Cell Sci

Rapid affinity purification of intracellular organelles using a twin strep tag.

Authors - Jian Xiong


Investigation of Glycosylphosphatidylinositol (GPI)-Plasma Membrane Interaction in Live Cells and the Influence of GPI Glycan Structure on the Interaction

Authors - Sayan Kundu

J Cancer

GP73 level determines chemotherapeutic resistance in human hepatocellular carcinoma cells.

Authors - Jia-Zhou Ye
  • KD Validated

Curr Alzheimer Res

RCAN1 Increases Aβ Generation by Promoting N-Glycosylation Via Oligosaccharyl Transferase.

Authors - T Wang

Eur Rev Med Pharmacol Sci

LncRNA MALAT1 knockdown inhibits cell migration and invasion by suppressing autophagy through miR-384/GOLM1 axis in glioma.

Authors - R Ma