GNA13 Monoclonal antibody

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  WB analysis using 67188-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67188-1-Ig (GNA13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67188-1-Ig

  WB result of GNA13 antibody (67188-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GNA13 transfected HEK-293 cells.

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  IHC staining of human breast cancer using 67188-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67188-1-Ig (GNA13 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67188-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67188-1-Ig (GNA13 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67188-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67188-1-Ig (GNA13 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67188-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67188-1-Ig (GNA13 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 67188-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using GNA13 antibody (67188-1-Ig, Clone: 2G8F10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• Featured Product
• KD/KO Validated

GNA13 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67188-1-Ig

Clone No.2G8F10

Publications(6)

Reviews (1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Synonyms

G alpha 13, G alpha-13, G protein subunit alpha 13, G13, G-protein subunit alpha-13

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Tested Applications

• Positive WB detected in: HEK-293 cells, Y79 cells, HepG2 cells, PC-12 cells, pig brain tissue, rat brain tissue, mouse brain tissue
• Positive IHC detected in: human breast cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• CoIP: See 1 publications below

Product Information

67188-1-Ig targets GNA13 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7316

  Product name: Recombinant human GNA13 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-377 aa of BC036756

  Sequence: MADFLPSRSVLSVCFPGCLLTSGEAEQQRKSKEIDKCLSREKTYVKRLVKILLLGAGESGKSTFLKQMRIIHGQDFDQRAREEFRPTIYSNVIKGMRVLVDAREKLHIPWGDNSNQQHGDKMMSFDTRAPMAAQGMVETRVFLQYLPAIRALWADSGIQNAYDRRREFQLGESVKYFLDNLDKLGEPDYIPSQQDILLARRPTKGIHEYDFEIKNVPFKMVDVGGQRSERKRWFECFDSVTSILFLVSSSEFDQVLMEDRLTNRLTESLNIFETIVNNRVFSNVSIILFLNKTDLLEEKVQIVSIKDYFLEFEGDPHCLRDVQKFLVECFRNKRRDQQQKPLYHHFTTAINTENIRLVFRDVKDTILHDNLKQLMLQ

• Full Name: guanine nucleotide binding protein (G protein), alpha 13
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC036756
• Gene Symbol: GNA13
• Gene ID (NCBI): 10672
• RRID: AB_2882483
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q14344
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Nat Commun; Integrin β3 directly inhibits the Gα13-p115RhoGEF interaction to regulate G protein signaling and platelet exocytosis; Authors - Yaping Zhang
human	WB,CoIP	J Proteome Res; Proteomic Investigation of Differential Interactomes of Glypican 1 and a Putative Disease-Modifying Variant of Ataxia; Authors - Nur Cengiz Winter
human	WB	J Clin Invest; Neomorphic Gαo mutations gain interaction with Ric8 proteins in GNAO1 encephalopathies; Authors - Gonzalo P Solis
human	WB	bioRxiv; The Adhesion GPCR ADGRL2 engages Ga13 to Enable Epidermal Differentiation; Authors - Xue Yang ; KD Validated
mouse	WB	Cell Mol Life Sci; GPR35 prevents drug-induced liver injury via the Gαs-cAMP-PKA axis in macrophages; Authors - Xueqin Zhao
human	WB	Adv Sci (Weinh); Hypoxia-Induced circPRELID2 Promotes Gastric Cancer Metastasis by Facilitating ZEB2 Translation via PCBP1 O-GlcNAcylation; Authors - Pengshan Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (12-29-2023)

Worked well for WB at 1:1000 in Glioma cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Glioma