GLRA2 Polyclonal antibody

GLRA2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 13831-1-AP


GLR, GLRA2, glycine receptor, alpha 2

Tested Applications

Positive WB detected inPBMC cells, K-562 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13831-1-AP targets GLRA2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GLRA2 fusion protein Ag4743
Full Name glycine receptor, alpha 2
Calculated Molecular Weight 452 aa, 52 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC032864
Gene Symbol GLRA2
Gene ID (NCBI) 2742
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycine is an inhibitory neurotransmitter in the central nervous system. The glycine receptor (GlyR) is a member of the nicotinic acetylcholine receptor of ligand-gated ion channels. This receptor has important roles in a variety of physiological processes, especially in mediating inhibitory neurotransmission in the spinal cord and brain stem. GlyR is a pentameric receptor comprised of alpha and beta subunits. Four alpha-subunits (GLRA1, GLRA2, GLRA3, GLRA4) and one beta-subunit (GLRB) of GlyR have been identified. (PMID: 11409700; 15383648)


Product Specific Protocols
WB protocol for GLRA2 antibody 13831-1-APDownload protocol
IHC protocol for GLRA2 antibody 13831-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Chelsea (Verified Customer) (02-17-2023)

We transfected HEK Cells with Glycine Receptor Alpha 1-2-3 to validate the GlyRa2 antibody. However, we saw staining in all conditions, even non transfected HEK cells (which have been validated to not contain endogenous GlyRa2). Therefore, we assume that the GlyRa2 antibody is not specific and binds an aspecifiek endogenous molecule.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Transfected HEK Cells