GLRA2 Polyclonal antibody

GLRA2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 13831-1-AP

Synonyms

GLR, GLRA2, glycine receptor, alpha 2

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Validation Data Gallery

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  WB analysis of PBMC cells using 13831-1-AP

  WB result of GLRA2 antibody (13831-1-AP, 1:600) with PBMC cells. Proteintech αGLRA2 13831-1-AP in 10% BSA / TBS 0.05% Tween-20 O/N @ 4°C Goat anti-rabbit HRP 1:10000 in 5% milk / TBS 0.05% Tween-20 1h @ RT.

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  WB analysis using 13831-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13831-1-AP (GLRA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 13831-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13831-1-AP (GLRA2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse brain using 13831-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13831-1-AP (GLRA2 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: PBMC cells, K-562 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13831-1-AP targets GLRA2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GLRA2 fusion protein Ag4743
• Full Name: glycine receptor, alpha 2
• Calculated Molecular Weight: 452 aa, 52 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC032864
• Gene Symbol: GLRA2
• Gene ID (NCBI): 2742
• RRID: AB_2877980
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycine is an inhibitory neurotransmitter in the central nervous system. The glycine receptor (GlyR) is a member of the nicotinic acetylcholine receptor of ligand-gated ion channels. This receptor has important roles in a variety of physiological processes, especially in mediating inhibitory neurotransmission in the spinal cord and brain stem. GlyR is a pentameric receptor comprised of alpha and beta subunits. Four alpha-subunits (GLRA1, GLRA2, GLRA3, GLRA4) and one beta-subunit (GLRB) of GlyR have been identified. (PMID: 11409700; 15383648)

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chelsea (Verified Customer) (02-17-2023)

We transfected HEK Cells with Glycine Receptor Alpha 1-2-3 to validate the GlyRa2 antibody. However, we saw staining in all conditions, even non transfected HEK cells (which have been validated to not contain endogenous GlyRa2). Therefore, we assume that the GlyRa2 antibody is not specific and binds an aspecifiek endogenous molecule.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Transfected HEK Cells