GLRA1 Polyclonal antibody

GLRA1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 17951-1-AP

Synonyms

GLRA1, glycine receptor, alpha 1, STHE

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Validation Data Gallery

• 

  WB analysis of mouse brain using 17951-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17951-1-AP (GLRA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 17951-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17951-1-AP (GLRA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 17951-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 17951-1-AP (GLRA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17951-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17951-1-AP (GLRA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse cerebellum tissue, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

17951-1-AP targets GLRA1 in WB, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GLRA1 fusion protein Ag12431
• Full Name: glycine receptor, alpha 1
• Calculated Molecular Weight: 449 aa, 52 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC074980
• Gene Symbol: GLRA1
• Gene ID (NCBI): 2741
• RRID: AB_2110060
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycine is an inhibitory neurotransmitter in the central nervous system. The glycine receptor (GlyR) is a member of the nicotinic acetylcholine receptor of ligand-gated ion channels. This receptor has important roles in a variety of physiological processes, especially in mediating inhibitory neurotransmission in the spinal cord and brain stem. GlyR is a pentameric receptor comprised of alpha and beta subunits. Four alpha-subunits (GLRA1, GLRA2, GLRA3, GLRA4) and one beta-subunit (GLRB) of GlyR have been identified. (PMID: 11409700; 15383648)

Publications

Species	Application	Title
mouse	CoIP	PLoS Biol; mGluR5/ERK signaling regulated the phosphorylation and function of glycine receptor α1ins subunit in spinal dorsal horn of mice.; Authors - Zi-Yang Zhang
mouse	WB,IF	J Biol Chem; Clinical, genetic, and functional characterization of the glycine receptor β-subunit A455P variant in a family affected by hyperekplexia syndrome.; Authors - Ghada I Aboheimed
mouse	WB	Psychopharmacology (Berl); Contribution of skeletal muscular glycine to rapid antidepressant effects of ketamine in an inflammation-induced mouse model of depression.; Authors - Niannian Huang
mouse	WB	Eur J Pharmacol; Functional expression of glycine receptors in DRG neurons of mice.; Authors - Lin Yao
human	WB	Neuropharmacology; Ubiquitination and inhibition of glycine receptor by HUWE1 in spinal cord dorsal horn.; Authors - Zi-Yang Zhang