GK5 Polyclonal antibody

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• KD/KO Validated

GK5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 13457-1-AP

Synonyms

GK 5, GK5, Glycerokinase 5, glycerol kinase 5 (putative), Putative glycerol kinase 5

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Validation Data Gallery

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  WB analysis of Jurkat using 13457-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13457-1-AP (GK5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

13457-1-AP targets GK5 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GK5 fusion protein Ag4269
• Full Name: glycerol kinase 5 (putative)
• Calculated Molecular Weight: 529 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC032470
• Gene Symbol: GK5
• Gene ID (NCBI): 256356
• RRID: AB_2111453
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GK5(Putative glycerol kinase 5) is also named as ATP:glycerol 3-phosphotransferase 5 and belongs to the FGGY kinase family.It is involved in Glycerol metabolism and catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate.It has 3 isoforms produced by alternative splicing with the MW of 59 kDa, 34 kDa and 28 kDa.

Publications

Species	Application	Title
human	WB	Clin Exp Pharmacol Physiol; Exposure to particulate matter 2.5 and cigarette smoke induces the synthesis of lipid droplets by glycerol kinase 5.; Authors - Mengnan Yan
human	WB,IF	J Exp Clin Cancer Res; PMID: 30791926; Authors - Jian Zhou ; KD Validated