GINS3 Polyclonal antibody

GINS3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 15651-1-AP

Synonyms

GINS complex subunit 3, GINS3, PSF3

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Validation Data Gallery

• 

  WB analysis of Jurkat using 15651-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15651-1-AP (GINS3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 15651-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15651-1-AP (GINS3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 15651-1-AP

  IP result of anti-GINS3 (IP:15651-1-AP, 4ug; Detection:15651-1-AP 1:300) with Jurkat cells lysate 3400ug.

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Tested Applications

• Positive WB detected in: Jurkat cells, NIH/3T3 cells
• Positive IP detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

15651-1-AP targets GINS3 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GINS3 fusion protein Ag8247
• Full Name: GINS complex subunit 3 (Psf3 homolog)
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC005879
• Gene Symbol: GINS3
• Gene ID (NCBI): 64785
• RRID: AB_2247477
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Mol Cell; Polε Instability Drives Replication Stress, Abnormal Development, and Tumorigenesis.; Authors - Roberto Bellelli
human	WB	J Clin Invest; Inherited GINS1 deficiency underlies growth retardation along with neutropenia and NK cell deficiency.; Authors - Julien Cottineau
human	WB	JCI Insight; Partial loss-of-function mutations in GINS4 lead to NK cell deficiency with neutropenia; Authors - Matilde I. Conte
human	WB	Cell Rep; Loss of POLE3-POLE4 unleashes replicative gap accumulation upon treatment with PARP inhibitors; Authors - Bethany Rebekah Hill