ChromoTek GFP RecAb, VHH-rabbit IgG Fc fusion

Rabbit monoclonal antibody to GFP. Recombinant fusion of anti-GFP VHH [CTK0201] and rabbit IgG Fc domain.

Host/IsoType

Rabbit/IgG

Specificity

GFP and GFP derivates

Applications

IF

Conjugate

Unconjugated

Clone No.

CTK0201

Cat no : gfrb

Synonyms

GFP, GFP tag, GFP-tag

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Validation Data Gallery

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  HeLa cells transiently expressing Mannosidase II-EGFP were immunostained with GFP recombinant antibody, VHH-rabbit IgG Fc fusion [CTK0201] (gfrb-20, 1:1,000) and Nano-Secondary? alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor? 647 [CTK0101, CTK0102] (srbAF647-1, 1:1,000). Scale bar, 10 μM.

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Product Information

Rabbit monoclonal antibody to GFP. Recombinant fusion of anti-GFP VHH [CTK0201] and rabbit IgG Fc domain.

• Applications: IF
• Host/IsoType: Rabbit/IgG
• Species Specificity: Recombinant protein from Aequorea victoria
• Specificity/Target: Green fluorescent protein (GFP) and derivates thereof such as Citrine, CFP, EGFP, EYFP, TagGFP, Venus, YFP
• Conjugate: Unconjugated
• Recommended Dilution: IF/ICC: 1:400 - 1:2,000
• GenBank Accession Number: U17997
• Purification Method: Recombinantly expressed and purified by IMAC
• Type: Primary antibody; chimeric fusion of Nanobody (VHH) and rabbit Fc domain
• Class: Recombinant
• Format: Recombinant monoclonal rabbit antibody
• Immunogen: Purified recombinant GFP [Aequorea victoria]
• Clonality: Monoclonal
• Clone No.: CTK0201
• Calculated MW: 77.6 kDa
• Form: Buffered aqueous solution
• RRID: AB_2889367
• Storage Buffer: PBS, 10% glycerol, Preservative: 0.02 % sodium azide, safety datasheet (SDS): sodium azide
• Storage Condition: Shipped on ice. Aliquot upon receipt and store at -20°C/-4°F. Avoid freeze-thaw cycles.
• Size: 20 µL; 100 µL