ChromoTek rat anti GFP Monoclonal antibody (3H9)

Rat monoclonal antibody [3H9] to Green Fluorescent Proteins

Host/IsoType

Rat/IgG2a

Specificity

GFP and GFP derivates

Applications

WB

Conjugate

Unconjugated

Clone No.

3H9

Publications

354

Reviews (3)

Cat no : 3h9

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet

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Validation Data Gallery

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  Dilution series of EGFP in HEK 293T cell lysate Primary antibody: [3H9] 1:1,000 Secondary antibody: goat anti-rat Alexa Fluor® 488 1:1,000

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Product Information

Rat monoclonal antibody [3H9] to Green Fluorescent Proteins

• Description: GFP rat monoclonal antibody [3H9] for WB
• Applications: WB
• Specificity/Target: The antibody recognizes green fluorescent proteins derived from Aequorea victoria GFP, such as CFP, EGFP, wtGFP, YFP, GFP.; For the complete list, please click here: Fluorescent protein specificity table antibodies
• Recommended Dilution: Western Blot: 1:1,000; Note: GFP antibody [3H9] does not work in immunofluorescence.
• Host/IsoType: Rat/IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Green Fluorescent Protein (GFP)
• UniProtKB: P42212
• Clone No.: 3H9
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A or G purified
• RRID: AB_10773374
• Storage Buffer: PBS, 50% glycerol, preservative: 0.09% sodium azide.
• Storage Condition: Shipped on ice. Store at -20°C/-4°F. Stable for 1 year after shipment.
• Background: Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.; When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.; This antibody is a rat monoclonal antibody against GFP, reactive to wtGFP, EGFP, YFP, and CFP.

Publications

Application	Title
WB	Nature; A pathway coordinated by DELE1 relays mitochondrial stress to the cytosol.; Authors - Evelyn Fessler
WB	Nature; TMK-based cell-surface auxin signalling activates cell-wall acidification.; Authors - Wenwei Lin
IP	Cell; A family of conserved bacterial virulence factors dampens interferon responses by blocking calcium signaling.; Authors - Noémie Alphonse
IF	Cell; The Ubiquitin Ligase CHIP Integrates Proteostasis and Aging by Regulation of Insulin Receptor Turnover.; Authors - Riga Tawo
WB	Cell; Exome capture reveals ZNF423 and CEP164 mutations, linking renal ciliopathies to DNA damage response signaling.; Authors - Moumita Chaki
IF	Nat Methods; Quantitative mapping and minimization of super-resolution optical imaging artifacts.; Authors - Siân Culley

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alexander (Verified Customer) (10-10-2024)

Did detect my construct wonderfully

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Benthamiana leave protein extraction

FH

Matthie (Verified Customer) (04-22-2024)

Very good signal even at low dilution. Works best on proteins denatured at high temperatures. No background signal.

• Applications: Western Blot
• Primary Antibody Dilution: 1/5000
• Cell Tissue Type: Yeast

FH

Andrea (Verified Customer) (09-19-2023)

The antibody works well, strong signal, clear background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:250