ChromoTek GFP VHH, recombinant binding protein

Alpaca anti-GFP VHH, purified recombinant binding protein

Specificity

GFP and GFP derivates

Applications

Conjugation

Type

Nanobody

Conjugate

Unconjugated

Publications

35

Cat no : gt

Synonyms

GFP-Binding-Protein, GFP-Nanobody, and GFP-single domain antibody (sdAb)

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Validation Data Gallery

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  Structure of GFP-VHH.

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Product Information

Alpaca anti-GFP VHH, purified recombinant binding protein

• Description: Alpaca anti-GFP VHH, purified recombinant binding protein for extraordinary stable & reliable binding

  • GFP-VHH:GFP complex is stable up to 80 °C, 1 mM DTT, 3 M Guanidinium•HCl, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % SDS, 1 % Triton X-100, 3 % Deoxycholate

  • Fulfills highest requirements for antibody validation

  • Structure and function are characterized

• Applications: Suitable for conjugation of dyes, biotin, beads, surfaces etc. via NHS ester reaction
• Specificity/Target: CFP, eGFP, wtGFP, GFP S65T, AcGFP, TagGFP, tagGFP2, sfGFP, pHluorin, eYFP, YFP, Venus, Citrine
• Conjugate: Unconjugated
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): Dissociation constant K_D of 1 pM
• Molecular Weight: 13.9 kDa
• RRID: AB_2631361
• Storage Buffer: 25mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, preservative: 0.09 % sodium azide
• Storage Condition: Shipped at ambient temperature. Upon receipt store at 4°C; stable for one year. Do not freeze.
• Background: Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.; When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.; This Nanobody is an alpaca recombinant VHH raised against GFP, reactive to a variety of GFP derivates, including CFP, eGFP, yeast eGFP, Clover, wtGFP, GFP S65T, AcGFP, TagGFP, tagGFP2, sfGFP, pHluorin, eYFP, YFP, Venus, YPET, Citrine and eCitrine and more.; For a full list of specificities see: Fluorescent_Specificity_by_Nano-Trap_190222.pdf (ptglab.com)

Publications

Application	Title
	Nat Methods; DART.2: bidirectional synaptic pharmacology with thousandfold cellular specificity; Authors - Brenda C Shields
IF	Nature; TORC1 organized in inhibited domains (TOROIDs) regulate TORC1 activity.; Authors - Manoël Prouteau
IF	Cell; Single-Molecule Nanoscopy Elucidates RNA Polymerase II Transcription at Single Genes in Live Cells.; Authors - Jieru Li
	Nat Methods; Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging.; Authors - Yongdeng Zhang
IF	Nat Protoc; Use of fluorescence-detected sedimentation velocity to study high-affinity protein interactions.; Authors - Sumit K Chaturvedi
	Trends Biotechnol; Nanobody-based products as research and diagnostic tools.; Authors - Thomas De Meyer