ChromoTek GFP VHH, biotinylated recombinant binding protein

Alpaca anti-GFP VHH, purified recombinant binding protein, conjugated to biotin. Compatible with avidin, streptavidin and neutravidin reagents.
Cat No. gtb

Specificity

GFP and GFP derivates

Applications

ELISA, SPR

Type

Nanobody

Conjugate

Biotin

Biotinylated GFP-Binding-Protein, biotinylated GFP-Nanobody, biotinylated anti-GFP camelid single-domain antibody fragment and biotinylated GFP-single domain antibody (sdAb)

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Product Information

Alpaca anti-GFP VHH, purified recombinant binding protein, conjugated to biotin. Compatible with avidin, streptavidin and neutravidin reagents.

DescriptionAlpaca anti-GFP VHH, purified recombinant binding protein, conjugated to biotin for extraordinary stable & reliable binding

• GFP-VHH:GFP complex is stable up to 80 °C, 1 mM DTT, 3 M Guanidinium•HCl, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % SDS, 1 % Triton X-100, 3 % Deoxycholate

• Fulfills highest requirements for antibody validation

• Structure and function are characterized

ApplicationsELISA
Surface Plasmon Resonance (SPR)
coating of multiwell plates
Specificity/TargetCFP, eGFP, wtGFP, GFP S65T, AcGFP, TagGFP, tagGFP2, sfGFP, pHluorin, eYFP, YFP, Venus, Citrine
For the complete list, please click here: Fluorescent protein specificity table
ConjugateBiotin (PEG4-Biotin)
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 1 pM
RRIDAB_2827557
Storage BufferBuffered aqueous solution (25 mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA), Preservative: 0.09% Sodium azide
Storage ConditionShipped at ambient temperature. Store at +4°C / 40°F. Stable for one year. Do not freeze.
Size250 µg

Publications

ApplicationTitle
IP

Nature

cAMP binding to closed pacemaker ion channels is non-cooperative.

Authors - David S White

Nat Nanotechnol

Oligomeric organization of membrane proteins from native membranes at nanoscale spatial and single-molecule resolution

Authors - Gerard Walker
BLI

Nat Commun

Targeting the actin nucleation promoting factor WASp provides a therapeutic approach for hematopoietic malignancies.

Authors - Guy Biber

Nat Commun

Single-molecule imaging with cell-derived nanovesicles reveals early binding dynamics at a cyclic nucleotide-gated ion channel.

Authors - Vishal R Patel
IP

J Cell Biol

Aurora B switches relative strength of kinetochore-microtubule attachment modes for error correction.

Authors - Harinath Doodhi
IF

PLoS Pathog

Poxviruses package viral redox proteins in lateral bodies and modulate the host oxidative response.

Authors - Susanna R. Bidgood