ChromoTek GFP VHH, biotinylated recombinant binding protein

Alpaca anti-GFP VHH, purified recombinant binding protein, conjugated to biotin. Compatible with avidin, streptavidin and neutravidin reagents.

Specificity

GFP and GFP derivates

Applications

ELISA, SPR

Type

Nanobody

Conjugate

Biotin

Publications

12

Cat no : gtb

Synonyms

Biotinylated GFP-Binding-Protein, biotinylated GFP-Nanobody, biotinylated anti-GFP camelid single-domain antibody fragment and biotinylated GFP-single domain antibody (sdAb)

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Validation Data Gallery

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  Structure of VHH.

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Product Information

Alpaca anti-GFP VHH, purified recombinant binding protein, conjugated to biotin. Compatible with avidin, streptavidin and neutravidin reagents.

• Description: Alpaca anti-GFP VHH, purified recombinant binding protein, conjugated to biotin for extraordinary stable & reliable binding

  • GFP-VHH:GFP complex is stable up to 80 °C, 1 mM DTT, 3 M Guanidinium•HCl, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % SDS, 1 % Triton X-100, 3 % Deoxycholate

  • Fulfills highest requirements for antibody validation

  • Structure and function are characterized

• Applications: ELISA; Surface Plasmon Resonance (SPR); coating of multiwell plates
• Specificity/Target: CFP, eGFP, wtGFP, GFP S65T, AcGFP, TagGFP, tagGFP2, sfGFP, pHluorin, eYFP, YFP, Venus, Citrine
• Conjugate: Biotin (PEG4-Biotin)
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): Dissociation constant K_D of 1 pM
• RRID: AB_2827557
• Storage Buffer: Buffered aqueous solution (25 mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA), Preservative: 0.09% Sodium azide
• Storage Condition: Shipped at ambient temperature. Store at +4°C / 40°F. Stable for one year. Do not freeze.

Publications

Application	Title
IP	Nature; cAMP binding to closed pacemaker ion channels is non-cooperative.; Authors - David S White
	Nat Nanotechnol; Oligomeric organization of membrane proteins from native membranes at nanoscale spatial and single-molecule resolution; Authors - Gerard Walker
BLI	Nat Commun; Targeting the actin nucleation promoting factor WASp provides a therapeutic approach for hematopoietic malignancies.; Authors - Guy Biber
	Nat Commun; Single-molecule imaging with cell-derived nanovesicles reveals early binding dynamics at a cyclic nucleotide-gated ion channel.; Authors - Vishal R Patel
IP	J Cell Biol; Aurora B switches relative strength of kinetochore-microtubule attachment modes for error correction.; Authors - Harinath Doodhi
IF	PLoS Pathog; Poxviruses package viral redox proteins in lateral bodies and modulate the host oxidative response.; Authors - Susanna R. Bidgood