GEMIN4 Polyclonal antibody

GEMIN4 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 12408-1-AP

Synonyms

GEMIN4, HC56, HCAP1, HHRF 1, p97

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Validation Data Gallery

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  WB analysis of HEK-293 using 12408-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12408-1-AP (GEMIN4 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12408-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12408-1-AP (GEMIN4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 cells using 12408-1-AP

  IP result of anti-GEMIN4 (12408-1-AP for IP and Detection) with 293 cell lysate.

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  IP experiment of HeLa using 12408-1-AP

  IP result of anti-GEMIN4 (IP:12408-1-AP, 5ug; Detection:12408-1-AP 1:200) with HeLa cells lysate 3100ug.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IP detected in: HEK-293 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12408-1-AP targets GEMIN4 in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GEMIN4 fusion protein Ag3099
• Full Name: gem (nuclear organelle) associated protein 4
• Calculated Molecular Weight: 1058 aa, 120 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: BC020062
• Gene Symbol: GEMIN4
• Gene ID (NCBI): 50628
• RRID: AB_10640891
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GEMIN4, also named as P97, is a sub-unit of SMN complex. The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs). It plays an important role in the splicing of cellular pre-mRNAs. The express amount of GEMIN4 is about 8.79 ppm (http://pax-db.org/#!protein/984742). This antibody detects 97-110 kDa GEMIN4.