CoraLite® Plus 488-conjugated GDF5 Recombinant antibody

GDF5 Uni-rAb^TM Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240944E5

Cat no : CL488-83988-4

Synonyms

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Validation Data Gallery

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  IF Staining of RAW 264.7 using CL488-83988-4

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CoraLite® Plus 488 GDF5 antibody (CL488-83988-4, Clone: 240944E5 ) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

• Positive IF/ICC detected in: RAW 264.7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-83988-4 is GDF5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GDF5 fusion protein Ag6238
• Full Name: growth differentiation factor 5
• Calculated Molecular Weight: 55 kDa
• GenBank Accession Number: BC032495
• Gene Symbol: GDF5
• Gene ID (NCBI): 8200
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Growth and Differentiation Factor 5 (GDF5) plays important roles during joint formation. It is one of the earliest genes expressed in the embryonic joint interzone7-10, fated to give rise to joint tissues including articular cartilage, synovium, menisci, and ligaments.