GCNT1 Recombinant antibody

GCNT1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240071E9

Cat no : 83121-4-RR

Synonyms

Core 2 beta-1,6-N-acetylglucosaminyltransferase, C2GNT-L, C2GNT1, C2GlcNAcT, Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase

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Validation Data Gallery

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  WB analysis using 83121-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83121-4-RR (GCNT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83121-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83121-4-RR against Human GCNT1 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: LNCaP cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83121-4-RR is GCNT1 Fusion Protein expressed in E. coli.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GCNT1 fusion protein Ag12264
• Full Name: glucosaminyl (N-acetyl) transferase 1, core 2 (beta-1,6-N-acetylglucosaminyltransferase)
• Calculated Molecular Weight: 428 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC074885
• Gene Symbol: GCNT1
• Gene ID (NCBI): 2650
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glucosaminyl (N-acetyl) transferase 1 (GCNT1, also known as C2GNT1), is a glycosyltransferase enzyme that plays an essential role in the formation of core 2 branched O-glycans that is crucial to the final definition of O-glycan structure (PMID: 37813880). GCNT1 promotes the growth of prostate tumors and regulates oncogenic gene expression pathways that are important for disease progression (PMID: 24854630).