GBF1 Polyclonal antibody

• Featured Product
• KD/KO Validated

GBF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 25183-1-AP

Synonyms

ARF1GEF, BFA resistant GEF 1, GBF1, KIAA0248

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Validation Data Gallery

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  WB analysis of HeLa using 25183-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25183-1-AP (GBF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 25183-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25183-1-AP (GBF1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 25183-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25183-1-AP (GBF1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 25183-1-AP

  Immunofluorescent analysis of HeLa cells using 25183-1-AP (GBF1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Product Information

The immunogen of 25183-1-AP is GBF1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GBF1 fusion protein Ag16579
• Full Name: golgi-specific brefeldin A resistant guanine nucleotide exchange factor 1
• Calculated Molecular Weight: 1859 aa, 206 kDa
• Observed Molecular Weight: 206 kDa
• GenBank Accession Number: BC117681
• Gene Symbol: GBF1
• Gene ID (NCBI): 8729
• RRID: AB_2879948
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; TBC1D23 mediates Golgi-specific LKB1 signaling; Authors - Yingfeng Tu
human	WB	PLoS Pathog; Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication.; Authors - Tao Wang
human	WB	Sci Rep; The Golgi protein ACBD3 facilitates Enterovirus 71 replication by interacting with 3A.; Authors - Xiaobo Lei ; KD Validated
human	IF	J Virol; Enterovirus 3A protein disrupts endoplasmic reticulum homeostasis through interaction with GBF1; Authors - Junki Hirano