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GBF1 Polyclonal antibody
GBF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 25183-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells |
Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 3 publications below |
Product Information
The immunogen of 25183-1-AP is GBF1 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | GBF1 fusion protein Ag16579 |
Full Name | golgi-specific brefeldin A resistant guanine nucleotide exchange factor 1 |
Calculated Molecular Weight | 1859 aa, 206 kDa |
Observed Molecular Weight | 206 kDa |
GenBank Accession Number | BC117681 |
Gene Symbol | GBF1 |
Gene ID (NCBI) | 8729 |
RRID | AB_2879948 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for GBF1 antibody 25183-1-AP | Download protocol |
IHC protocol for GBF1 antibody 25183-1-AP | Download protocol |
IF protocol for GBF1 antibody 25183-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
PLoS Pathog Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication. | ||
Sci Rep The Golgi protein ACBD3 facilitates Enterovirus 71 replication by interacting with 3A.
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