GATA4-Specific Polyclonal antibody

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GATA4-Specific Polyclonal Antibody for WB, ELISA

Cat No. 19530-1-AP Copied!

Publications (52)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, IP, CoIP, ChiP, ELISA

Synonyms

GATA binding factor 4, GATA binding protein 4, GATA4, GATA4-Specific, Transcription factor GATA 4

• 

  WB analysis of mouse liver using 19530-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19530-1-AP (GATA4-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 19530-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19530-1-AP (GATA4-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat heart using 19530-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 19530-1-AP (GATA4-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse heart using 19530-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19530-1-AP (GATA4-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse liver tissue, mouse heart tissue, rat heart tissue, PC-3 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19530-1-AP targets GATA4-Specific in WB, IHC, IF, IP, CoIP, ChiP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: GATA binding protein 4
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: NM_002052
• Gene Symbol: GATA4
• Gene ID (NCBI): 2626
• RRID: AB_10642003
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P43694
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

What is the molecular weight of GATA4? Is GATA4 post-translationally modified?

The molecular weight of GATA4 is 48 kDa. GATA4 can be phosphorylated, which regulates its activity (PMID: 10788492).

 What is the subcellular localization of GATA4?

GATA4 localizes to the nucleus. The nuclear localization motif is present in the C-terminal part of GATA4 (PMID: 9079680).

 What is the tissue expression pattern of GATA4?

GATA4 was initially identified as a cardiac transcription factor important for the development of the heart and as one of the earliest transcription factors expressed in developing cardiac cells. However, GATA4 is expressed in numerous organs such as the liver, lung, gonads, and small intestine (PMID: 8455608).

Publications

What published studies show

The anti-GATA4 antibody (Cat# 19530-1-AP) has 52 citations published across journals including Nature, Nature Communications, Molecular Cell, EMBO Journal, Genome Biology, Cell Death & Disease, Advanced Science, and PLoS ONE. Research fields span cardiac disease (hypertrophy, heart failure, myocardial infarction), cancer (pancreatic, ovarian, breast, nasopharyngeal), cellular senescence, stem cell differentiation, developmental biology, immunology, vascular biology, bone metabolism, and toxicology. Applications include WB, IF, IHC, CoIP, IP, and ChIP.

Automatically generated from the 52 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human,mouse	WB,IF	Nat Commun; Cell enlargement modulated by GATA4 and YAP instructs the senescence-associated secretory phenotype; Authors - Joae Joung
human	IF	Nat Commun; Regulation of senescence-associated secretory phenotypes in osteoarthritis by cytosolic UDP-GlcNAc retention and O-GlcNAcylation; Authors - Donghyun Kang
mouse	WB	ACS Nano; Silver Nanoparticles Compromise Female Embryonic Stem Cell Differentiation through Disturbing X Chromosome Inactivation.; Authors - Jie Zhang
human	WB	Mol Cell; Histone chaperone HIRA, promyelocytic leukemia protein, and p62/SQSTM1 coordinate to regulate inflammation during cell senescence.; Authors - Nirmalya Dasgupta
human	WB, CoIP	J Exp Clin Cancer Res; Overexpressed WDR3 induces the activation of Hippo pathway by interacting with GATA4 in pancreatic cancer.; Authors - Wenjie Su