GATA4-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

GATA4-Specific Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IF, IHC, CoIP, ChiP, ELISA

Conjugate

Unconjugated

Publications

35

Cat no : 19530-1-AP

Synonyms

GATA binding factor 4, GATA binding protein 4, GATA4, GATA4-Specific, Transcription factor GATA 4

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Validation Data Gallery

• 

  WB analysis of mouse liver using 19530-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19530-1-AP (GATA4-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19530-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19530-1-AP (GATA4-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 19530-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19530-1-AP (GATA4-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 19530-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 19530-1-AP (GATA4-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse liver tissue, mouse heart tissue, rat heart tissue, PC-3 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 24 publications below
• IHC: See 4 publications below
• IF: See 11 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 2 publications below

Product Information

19530-1-AP targets GATA4-Specific in WB, IP, IF, IHC, CoIP, ChiP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: GATA binding protein 4
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: NM_002052
• Gene Symbol: GATA4
• Gene ID (NCBI): 2626
• RRID: AB_10642003
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

What is the molecular weight of GATA4? Is GATA4 post-translationally modified?

The molecular weight of GATA4 is 48 kDa. GATA4 can be phosphorylated, which regulates its activity (PMID: 10788492).

 What is the subcellular localization of GATA4?

GATA4 localizes to the nucleus. The nuclear localization motif is present in the C-terminal part of GATA4 (PMID: 9079680).

 What is the tissue expression pattern of GATA4?

GATA4 was initially identified as a cardiac transcription factor important for the development of the heart and as one of the earliest transcription factors expressed in developing cardiac cells. However, GATA4 is expressed in numerous organs such as the liver, lung, gonads, and small intestine (PMID: 8455608).

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
mouse	WB,IHC,IF,IP	Clin Transl Med; Bmi-1-RING1B prevents GATA4-dependent senescence-associated pathological cardiac hypertrophy by promoting autophagic degradation of GATA4.; Authors - Haiyun Chen
human	IF	Elife; Induction of Sertoli-like cells from human fibroblasts by NR5A1 and GATA4.; Authors - Jianlin Liang
human	WB	Cardiovasc Res; Circular RNA circRNA_000203 aggravates cardiac hypertrophy via suppressing miR26b-5p and miR-140-3p binding to Gata4.; Authors - Hui Li
mouse	WB	J Bone Miner Res; Bmi-1 Overexpression Improves Sarcopenia Induced by 1,25(OH)2 D3 Deficiency and Downregulates GATA4-Dependent Rela Transcription; Authors - Qiuyi Wang
mouse	WB	Cell Prolif; Carbon monoxide alleviates senescence in diabetic nephropathy by improving autophagy.; Authors - Li Chen

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