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GATA4-Specific Polyclonal antibody

GATA4-Specific Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 19530-1-AP

Synonyms

GATA binding factor 4, GATA binding protein 4, GATA4, GATA4-Specific, Transcription factor GATA 4



Tested Applications

Positive WB detected inHepG2 cells, mouse liver tissue, mouse heart tissue, rat heart tissue, PC-3 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Sample-dependent, check data in validation data gallery

Product Information

19530-1-AP targets GATA4-Specific in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name GATA binding protein 4
Calculated molecular weight 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberNM_002052
Gene symbol GATA4
Gene ID (NCBI) 2626
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

What is the molecular weight of GATA4? Is GATA4 post-translationally modified?

The molecular weight of GATA4 is 48 kDa. GATA4 can be phosphorylated, which regulates its activity (PMID: 10788492).

 What is the subcellular localization of GATA4?

GATA4 localizes to the nucleus. The nuclear localization motif is present in the C-terminal part of GATA4 (PMID: 9079680).

 What is the tissue expression pattern of GATA4?

GATA4 was initially identified as a cardiac transcription factor important for the development of the heart and as one of the earliest transcription factors expressed in developing cardiac cells. However, GATA4 is expressed in numerous organs such as the liver, lung, gonads, and small intestine (PMID: 8455608).



Protocols

Product Specific Protocols
WB protocol for GATA4-Specific antibody 19530-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
mouseWB,IHC,IF,IP

Clin Transl Med

Bmi-1-RING1B prevents GATA4-dependent senescence-associated pathological cardiac hypertrophy by promoting autophagic degradation of GATA4.

Authors - Haiyun Chen
humanIF

Elife

Induction of Sertoli-like cells from human fibroblasts by NR5A1 and GATA4.

Authors - Jianlin Liang
humanWB

Cardiovasc Res

Circular RNA circRNA_000203 aggravates cardiac hypertrophy via suppressing miR26b-5p and miR-140-3p binding to Gata4.

Authors - Hui Li
mouseWB

J Bone Miner Res

Bmi-1 Overexpression Improves Sarcopenia Induced by 1,25(OH)2 D3 Deficiency and Downregulates GATA4-Dependent Rela Transcription

Authors - Qiuyi Wang
mouseWB

Cell Prolif

Carbon monoxide alleviates senescence in diabetic nephropathy by improving autophagy.

Authors - Li Chen