GAPDHS Polyclonal antibody

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  WB analysis using 13937-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13937-1-AP (GAPDHS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13937-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13937-1-AP (GAPDHS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

GAPDHS Polyclonal Antibody for WB, ELISA

Cat No. 13937-1-AP

Publications(16)

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, human and More (1)

Applications

WB, ELISA

Synonyms

GAPD2, GAPDH 2, GAPDH2, GAPDHS, GAPDS, HSD 35

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 15 publications below

Product Information

13937-1-AP targets GAPDHS in WB, ELISA applications and shows reactivity with mouse, rat, human samples.

• Tested Reactivity: mouse, rat, human
• Cited Reactivity: human, mouse, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4988

  Product name: Recombinant human GAPDHS protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 58-408 aa of BC036373

  Sequence: LPPHPATPPPKMVSVARELTVGINGFGRIGRLVLRACMEKGVKVVAVNDPFIDPEYMVYMFKYDSTHGRYKGSVEFRNGQLVVDNHEISVYQCKEPKQIPWRAVGSPYVVESTGVYLSIQAASDHISAGAQRVVISAPSPDAPMFVMGVNENDYNPGSMNIVSNASCTTNCLAPLAKVIHERFGIVEGLMTTVHSYTATQKTVDGPSRKAWRDGRGAHQNIIPASTGAAKAVTKVIPELKGKLTGMAFRVPTPDVSVVDLTCRLAQPAPYSAIKEAVKAAAKGPMAGILAYTEDEVVSTDFLGDTHSSIFDAKAGIALNDNFVKLISWYDNEYGYSHRVVDLLRYMFSRDK

• Full Name: glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC036373
• Gene Symbol: GAPDHS
• Gene ID (NCBI): 26330
• RRID: AB_2107611
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14556
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GAPDHS(Glyceraldehyde-3-phosphate dehydrogenase, testis-specific) is also named as GAPD2, GAPDH2, GAPDS and belongs to the glyceraldehyde-3-phosphate dehydrogenase family. It may play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. GAPDHS is a promising contraceptive target because it is specific to male germ cells, essential for sperm motility and male fertility, and well suited to pharmacological inhibition(PMID:20828617).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	J Hazard Mater; Multi-omics analyses reveal the mechanisms of Arsenic-induced male reproductive toxicity in mice.; Authors - Zijun Peng
human	WB	Theranostics; Phosphorylation of IRS4 by CK1γ2 promotes its degradation by CHIP through the ubiquitin/lysosome pathway.; Authors - Xinchun Li
human	WB	Theranostics; Up-regulation of PCOLCE by TWIST1 promotes metastasis in Osteosarcoma.; Authors - Shang Wang
mouse	WB	Oxid Med Cell Longev; Withaferin A Exerts Preventive Effect on Liver Fibrosis through Oxidative Stress Inhibition in a Sirtuin 3-Dependent Manner.; Authors - Jingya Gu
rat	WB	J Cell Mol Med; Celastrol exerts anti-inflammatory effect in liver fibrosis via activation of AMPK-SIRT3 signalling.; Authors - Yuqin Wang
human	WB	Front Cell Dev Biol; Sperm-Specific Glycolysis Enzyme Glyceraldehyde-3-Phosphate Dehydrogenase Regulated by Transcription Factor SOX10 to Promote Uveal Melanoma Tumorigenesis.; Authors - Xia Ding