GAC-specific Polyclonal antibody, PBS Only

GAC-specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 19958-1-PBS

Synonyms

GLS, KGA, GLS1, GAM, GAC

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Validation Data Gallery

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  WB analysis using 19958-1-AP (same clone as 19958-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19958-1-AP (GAC-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19958-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 19958-1-AP (same clone as 19958-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19958-1-AP (GAC-specific Antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 19958-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 19958-1-AP (same clone as 19958-1-PBS)

  IP result of anti-GAC-specific (IP:19958-1-AP, 3ug; Detection:19958-1-AP 1:500) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 19958-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 19958-1-AP (same clone as 19958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19958-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 19958-1-AP (same clone as 19958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19958-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 19958-1-AP (same clone as 19958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19958-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 19958-1-AP (same clone as 19958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19958-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 19958-1-AP (same clone as 19958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19958-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 19958-1-AP (same clone as 19958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19958-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 19958-1-AP (same clone as 19958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 19958-1-AP (GAC-specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 19958-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 19958-1-AP (same clone as 19958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 19958-1-AP (GAC-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 19958-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 19958-1-AP (same clone as 19958-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using GAC-specific antibody (19958-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 19958-1-PBS in a different storage buffer formulation.

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Product Information

19958-1-PBS targets GAC-specific in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: glutaminase
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: AF158555
• Gene Symbol: GLS
• Gene ID (NCBI): 2744
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Glutaminase C (GAC), a splicing variant of the kidney-type glutaminase (KGA) gene, is a vital mitochondrial enzyme protein that catalyzes glutamine to glutamate. GAC is the only isoform present in the mitochondria (PMID:22228304). In a normal state, immunodecorationwith antibodies directed against GAC identified an abundant complex of 232 kDa as a tetrameric form of GAC, the DPAA can directly destructed the native tetrameric GAC complex to accumulate the unassembled one (50-90 kDa). (PMID:22493432). This antibody is specific to GAC isoform. It has no cross reaction to KGA.