GABARAPL1-Specific Polyclonal antibody, PBS Only

GABARAPL1-Specific Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18721-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 18721-1-AP (same clone as 18721-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18721-1-AP (GABARAPL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18721-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 18721-1-AP (same clone as 18721-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18721-1-AP (GABARAPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18721-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 18721-1-AP (same clone as 18721-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 18721-1-AP (GABARAPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18721-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human spleen using 18721-1-AP (same clone as 18721-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 18721-1-AP (GABARAPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18721-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 18721-1-AP (same clone as 18721-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 18721-1-AP (GABARAPL1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18721-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 18721-1-AP (same clone as 18721-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 18721-1-AP (GABARAPL1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18721-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 18721-1-AP (same clone as 18721-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18721-1-AP (GABARAPL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18721-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 18721-1-AP (same clone as 18721-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18721-1-AP (GABARAPL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18721-1-PBS in a different storage buffer formulation.

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Product Information

18721-1-PBS targets GABARAPL1-Specific in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: GABA(A) receptor-associated protein like 1
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: NM_031412
• Gene Symbol: GABARAPL1
• Gene ID (NCBI): 23710
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GABARAPL1 (GABARAP-like protein 1), also named as ATG8, GEC1, APG8L, ATG8L and APG8-LIKE, is a member of GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as estrogen-regulated gene and the protein acts in receptor and vesicle transport. It's also involved in the process of autophagy like GABARAP and GABARAPL2, and may be considered as an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta and skeletal muscle. And at very low levels in thymus and small intestine.The antibody is specific to GABARAPL1, and it doesn't recognize the recombinant GABARAP and GABARAPL2.