GABARAPL1-Specific Polyclonal antibody

GABARAPL1-Specific Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 18721-1-AP

Synonyms

GABARAPL1, GABA(A) receptor-associated protein-like 1, Early estrogen-regulated protein, ATG8L, ATG8

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Validation Data Gallery

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  WB analysis using 18721-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18721-1-AP (GABARAPL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 18721-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18721-1-AP (GABARAPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 18721-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 18721-1-AP (GABARAPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 18721-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 18721-1-AP (GABARAPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 18721-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18721-1-AP (GABARAPL1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 18721-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18721-1-AP (GABARAPL1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse brain using 18721-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18721-1-AP (GABARAPL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 18721-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18721-1-AP (GABARAPL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HepG2 using 18721-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug GABARAPL1-Specific Polyclonal antibody (18721-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human heart tissue, human spleen tissue, mouse kidney tissue, mouse liver tissue, rat brain tissue
• Positive IHC detected in: human kidney tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

18721-1-AP targets GABARAPL1-Specific in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: GABA(A) receptor-associated protein like 1
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: NM_031412
• Gene Symbol: GABARAPL1
• Gene ID (NCBI): 23710
• RRID: AB_10597556
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GABARAPL1 (GABARAP-like protein 1), also named ATG8, GEC1, APG8L, ATG8L, and APG8-LIKE, is a member of the GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as an estrogen-regulated gene and the protein acts in receptor and vesicle transport. It's also involved in the process of autophagy like GABARAP and GABARAPL2 and may be considered as an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta, and skeletal muscle. And at very low levels in the thymus and small intestine. The antibody is specific to GABARAPL1 and doesn't recognize the recombinant GABARAP and GABARAPL2.

Publications

Species	Application	Title
human	WB	Autophagy; GABARAPL1 antibodies: target one protein, get one free!; Authors - Le Grand Jaclyn Nicole JN
human	WB	Sci Rep; Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization.; Authors - Alexandra Boeske
human	WB	BMC Musculoskelet Disord; MiR-145 inhibits the differentiation and proliferation of bone marrow stromal mesenchymal stem cells by GABARAPL1 in steroid-induced femoral head necrosis; Authors - Pengfei Xu