G6PD Polyclonal antibody

• Featured Product
• KD/KO Validated

G6PD Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

43

Cat no : 25413-1-AP

Synonyms

Glucose-6-phosphate 1-dehydrogenase, G6PD1, G6P, EC:1.1.1.49

---

Validation Data Gallery

• 

  WB analysis using 25413-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25413-1-AP (G6PD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25413-1-AP

  WB result of G6PD antibody (25413-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-G6PD transfected HeLa cells.

• 

  WB analysis of HeLa using 25413-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25413-1-AP (G6PD Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 25413-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25413-1-AP (G6PD Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25413-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25413-1-AP (G6PD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 25413-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25413-1-AP (G6PD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 25413-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25413-1-AP (G6PD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 25413-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25413-1-AP (G6PD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 25413-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25413-1-AP (G6PD antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 25413-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25413-1-AP (G6PD antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 25413-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using G6PD antibody (25413-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

---

Tested Applications

• Positive WB detected in: MCF-7 cells, HeLa cells, HEK-293 cells
• Positive IHC detected in: human liver cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 36 publications below
• IHC: See 8 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

25413-1-AP targets G6PD in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: G6PD fusion protein Ag21862
• Full Name: glucose-6-phosphate dehydrogenase
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC000337
• Gene Symbol: G6PD
• Gene ID (NCBI): 2539
• RRID: AB_2880066
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

G6PD(Glucose-6-phosphate 1-dehydrogenase) belongs to the glucose-6-phosphate dehydrogenase family. It is a housekeeping enzyme encoded in mammals by an X-linked gene. G6PD has important functions in intermediary metabolism because it catalyzes the first step in the pentose phosphate pathway and provides reductive potential in the form of NADPH.(PMID:7489710). This protein has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	IHC	Sci Adv; G6PD-mediated increase in de novo NADP+ biosynthesis promotes antioxidant defense and tumor metastasis.; Authors - Yang Zhang
human	WB, IP	Nat Commun; Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway.; Authors - Xiaoyu Ma
human	WB	Acta Pharmacol Sin; Targeting proteasomal deubiquitinases USP14 and UCHL5 with b-AP15 reduces 5-fluorouracil resistance in colorectal cancer cells; Authors - Wa Ding
human	WB	Phytomedicine; Tiliroside disrupted iron homeostasis and induced ferroptosis via directly targeting calpain-2 in pancreatic cancer cells; Authors - Maolei Xu
human	WB	Phytomedicine; Tiliroside targets TBK1 to induce ferroptosis and sensitize hepatocellular carcinoma to sorafenib; Authors - Chen Yang
human,mouse	WB,IHC	Oxid Med Cell Longev; Cigarette Smoke Exposure Increases Glucose-6-phosphate Dehydrogenase, Autophagy, Fibrosis, and Senescence in Kidney Cells In Vitro and In Vivo.; Authors - Wen-Chih Liu