G6PC Polyclonal antibody

G6PC Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 22169-1-AP

Synonyms

G 6 Pase, G6P, G6Pase, G6Pase alpha, G6PC, G6PT, Glucose 6 phosphatase, Glucose 6 phosphatase alpha, GSD1, GSD1a



Tested Applications

Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 2 publications below

Product Information

22169-1-AP targets G6PC in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen G6PC fusion protein Ag17839
Full Name glucose-6-phosphatase, catalytic subunit
Calculated Molecular Weight 357 aa, 40 kDa
GenBank Accession NumberBC130478
Gene Symbol G6PC
Gene ID (NCBI) 2538
RRIDAB_2879015
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glucose-6-phosphatase-α (G6PC) is a key enzyme in glucose homeostasis that catalyzes the hydrolysis of glucose-6-phosphate to glucose and phosphate in the terminal step of gluconeogenesis and glycogenolysis. G6PC activity is restricted to the liver , the kidney cortex and the small intestine and confers on these three organs the capacity to release glucose into the systemic circulation (PMID: 21983240).The encoded enzyme is anchored to the ER by nine transmembrane helices with the amino (N)-terminus in the lumen and the carboxyl (C)-terminus in the cytoplasm (PMID: 15542400).

Protocols

Product Specific Protocols
IHC protocol for G6PC antibody 22169-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

Mol Metab

Disrupted Liver Oxidative Metabolism in Glycine N-Methyltransferase-Deficient Mice is Mitigated by Dietary Methionine Restriction.

Authors - Ferrol I Rome
mouse

Mol Nutr Food Res

Douchi Peptides Vy and Sfllr Improve Glucose Homeostasis and Gut Dysbacteriosis In High-Fat Diet-Induced Insulin Resistant Mice

Authors - Songfeng Yu
mouse

J Genet Genomics

Functionalized Gadofullerene Ameliorates Impaired Glycolipid Metabolism in Type 2 Diabetic Mice.

Authors - Jin Wu
mouse

Endocrinology

Aspirin Suppresses Hepatic Glucagon Signaling Through Decreasing Production of Thromboxane A2

Authors - Yufeng Dai
human

Pharmacol Res

PMID: 29284152

Authors - Jiting Yan
mouse

Front Mol Biosci

Paternal High-Fat Diet Altered Sperm 5'tsRNA-Gly-GCC Is Associated With Enhanced Gluconeogenesis in the Offspring.

Authors - Bin Wang