PE Anti-Mouse FceR1a (MAR-1)

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  FC experiment of MC/9 using PE-65091

  1X10^6 MC/9 cells were surface stained with 0.13 ug PE Anti-Mouse FceR1 (PE-65091, Clone: MAR-1) (red) or 0.13 ug isotype control antibody (black). Cells were not fixed.

FceR1a Monoclonal Antibody for FC

Cat No. PE-65091

Clone No.MAR-1

Host / Isotype

Hamster / IgG

Reactivity

mouse

Applications

FC

Synonyms

Fce1a, FceR1, FcERI, Fcr 5, FcεRIα

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Tested Applications

• Positive FC detected in: MC/9 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.13 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

PE-65091 targets FceR1a in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  N/A

• Full Name: Fc receptor, IgE, high affinity I, alpha polypeptide
• GenBank Accession Number: BC125455
• Gene Symbol: FceR1a
• Gene ID (NCBI): 14125
• RRID: AB_2883885
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P20489
• Storage Buffer: PBS with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Fc epsilon Receptor I alpha (FceR1a) is a single-pass type I membrane protein that contains 2 immunoglobulin-like domains. FceR1a forms a tetrameric complex, FceRI, with one beta and two gamma subunits (PMID: 19406478). FceRI is the high-affinity receptor for the Fc region of immunoglobulin E (IgE) (PMID: 1532373). It is expressed on mast cells and basophils and plays a central role in the allergic response (PMID: 2148316).