CoraLite® Plus 488 Anti-Mouse FceR1a (MAR-1)

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  FC experiment of MC/9 using CL488-65091

  1X10^6 MC/9 cells were surface stained with 5 ul CoraLite® Plus 488 Anti-Mouse FceR1 (CL488-65091, Clone:MAR-1) (green) or CoraLite® Plus 488 Armenian Hamster IgG isotype control (black). Cells were not fixed.

FceR1a Monoclonal Antibody for FC

Cat No. CL488-65091

Clone No.MAR-1

Host / Isotype

Armenian Hamster / IgG

Reactivity

mouse

Applications

FC

Synonyms

MAR-1, High affinity immunoglobulin epsilon receptor subunit alpha, Fcr 5, FcERI, FceR1

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Tested Applications

• Positive FC detected in: MC/9 cells

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65091 targets FceR1a in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Armenian Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: Fc receptor, IgE, high affinity I, alpha polypeptide
• GenBank Accession Number: BC125455
• Gene Symbol: FceR1a
• Gene ID (NCBI): 14125
• RRID: AB_2919259
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Fc epsilon Receptor I alpha (FceR1a) is a single-pass type I membrane protein that contains 2 immunoglobulin-like domains. FceR1a forms a tetrameric complex, FceRI, with one beta and two gamma subunits (PMID: 19406478). FceRI is the high-affinity receptor for the Fc region of immunoglobulin E (IgE) (PMID: 1532373). It is expressed on mast cells and basophils and plays a central role in the allergic response (PMID: 2148316).