FZR1/Cdh1 Polyclonal antibody

• Featured Product
• KD/KO Validated

FZR1/Cdh1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (1)

Cat no : 16368-1-AP

Synonyms

FZR1, Fizzy related protein homolog, Cdh1/Hct1 homolog, CDH1, CDC20-like protein 1

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Validation Data Gallery

• 

  WB analysis using 16368-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16368-1-AP (FZR1/Cdh1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 16368-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16368-1-AP (FZR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16368-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16368-1-AP (FZR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 16368-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16368-1-AP (FZR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16368-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16368-1-AP (FZR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 16368-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 16368-1-AP (FZR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 16368-1-AP

  IP result of anti-FZR1/Cdh1 (IP:16368-1-AP, 4ug; Detection:16368-1-AP 1:500) with mouse heart tissue lysate 3200ug.

• 

  IHC staining of human liver using 16368-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16368-1-AP (FZR1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 16368-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16368-1-AP (FZR1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 16368-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16368-1-AP (FZR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16368-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16368-1-AP (FZR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16368-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 16368-1-AP (FZR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 16368-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16368-1-AP (FZR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 16368-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human FZR1/Cdh1 (16368-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HMy2.CIR cells, C6 cells, HepG2 cells, mouse heart tissue, mouse liver tissue, NIH/3T3 cells, HeLa cells, Jurkat cells
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human liver tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IP: See 2 publications below
• FC: See 1 publications below

Product Information

16368-1-AP targets FZR1/Cdh1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FZR1/Cdh1 fusion protein Ag9201
• Full Name: fizzy/cell division cycle 20 related 1 (Drosophila)
• Calculated Molecular Weight: 493 aa, 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC013413
• Gene Symbol: FZR1
• Gene ID (NCBI): 51343
• RRID: AB_2107380
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FZR1 gene, also named as FYR, FZR and KIAA1242, has been previously shown to be mutated in lobular breast carcinomas. Direct evidence of FZR1 mutations triggering tumorigenesis has been investigated. FZR1 is a key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C) which is playing vital role s in cell cycle progression. There are 7 WD-repeat domains in FZR1. Upon phosphorylation and dephosphorylation, it interacts with other components to modulate cell mitosis. This gene is different to E-cadherin (CDH1)which is an epithelial cell adhesion molecule. It has 55 kDa, 54 kDa and 45 kDa isoforms.

Publications

Species	Application	Title
human	WB	Nat Commun; DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation; Authors - Xiaohua Xu
mouse	WB	Sci Adv; The cohesin stabilizer Sororin drives G2-M transition and spindle assembly in mammalian oocytes.; Authors - Changyin Zhou ; KD Validated
human	WB	Acta Pharmacol Sin; Bergenin, a PPARγ agonist, inhibits Th17 differentiation and subsequent neutrophilic asthma by preventing GLS1-dependent glutaminolysis.; Authors - Ling Yang
mouse	WB	Mol Cell Biol; ERBB2 deficiency alters an E2F-1-dependent adaptive stress response and leads to cardiac dysfunction.; Authors - Marie-Claude Perry
	IP,WB	iScience; Cdh1-APC Regulates Protein Synthesis and Stress Granules in Neurons through an FMRP-Dependent Mechanism.; Authors - Arielle N Valdez-Sinon ; KD Validated
mouse	FC	Cell Death Dis; A Cdh1-FoxM1-Apc axis controls muscle development and regeneration.; Authors - Zhe Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chun (Verified Customer) (09-07-2020)

This antibody appears to be good for immunoblotting.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HaCaT and HEK293 cells

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