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FZR1/Cdh1 Polyclonal antibody
FZR1/Cdh1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Conjugate
Unconjugated
Cat no : 16368-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, C6 cells, HepG2 cells, Jurkat cells, mouse heart tissue, mouse liver tissue, NIH/3T3 cells |
Positive IP detected in | mouse heart tissue |
Positive IHC detected in | human liver tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 7 publications below |
IP | See 2 publications below |
FC | See 1 publications below |
Product Information
16368-1-AP targets FZR1/Cdh1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | FZR1/Cdh1 fusion protein Ag9201 |
Full Name | fizzy/cell division cycle 20 related 1 (Drosophila) |
Calculated Molecular Weight | 493 aa, 55 kDa |
Observed Molecular Weight | 55 kDa |
GenBank Accession Number | BC013413 |
Gene Symbol | FZR1 |
Gene ID (NCBI) | 51343 |
RRID | AB_2107380 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FZR1 gene, also named as FYR, FZR and KIAA1242, has been previously shown to be mutated in lobular breast carcinomas. Direct evidence of FZR1 mutations triggering tumorigenesis has been investigated. FZR1 is a key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C) which is playing vital role s in cell cycle progression. There are 7 WD-repeat domains in FZR1. Upon phosphorylation and dephosphorylation, it interacts with other components to modulate cell mitosis. This gene is different to E-cadherin (CDH1)which is an epithelial cell adhesion molecule. It has 55 kDa, 54 kDa and 45 kDa isoforms.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for FZR1/Cdh1 antibody 16368-1-AP | Download protocol |
IHC protocol for FZR1/Cdh1 antibody 16368-1-AP | Download protocol |
IF protocol for FZR1/Cdh1 antibody 16368-1-AP | Download protocol |
IP protocol for FZR1/Cdh1 antibody 16368-1-AP | Download protocol |
FC protocol for FZR1/Cdh1 antibody 16368-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation | ||
Sci Adv The cohesin stabilizer Sororin drives G2-M transition and spindle assembly in mammalian oocytes.
| ||
Acta Pharmacol Sin Bergenin, a PPARγ agonist, inhibits Th17 differentiation and subsequent neutrophilic asthma by preventing GLS1-dependent glutaminolysis. | ||
Mol Cell Biol ERBB2 deficiency alters an E2F-1-dependent adaptive stress response and leads to cardiac dysfunction. | ||
iScience Cdh1-APC Regulates Protein Synthesis and Stress Granules in Neurons through an FMRP-Dependent Mechanism.
| ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Chun (Verified Customer) (09-07-2020) | This antibody appears to be good for immunoblotting.
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