FURIN Monoclonal antibody, PBS Only

FURIN Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D1B9

Cat no : 67481-1-PBS

Synonyms

Dibasic processing enzyme, FUR, FURIN, PACE, PCSK3, SPC1

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Validation Data Gallery

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  WB analysis using 67481-1-Ig (same clone as 67481-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67481-1-Ig (FURIN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67481-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 67481-1-Ig (same clone as 67481-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 67481-1-Ig (FURIN antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67481-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67481-1-Ig (same clone as 67481-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67481-1-Ig (FURIN antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67481-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 67481-1-Ig (same clone as 67481-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67481-1-Ig (FURIN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67481-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 67481-1-Ig (same clone as 67481-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67481-1-Ig (FURIN antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67481-1-PBS in a different storage buffer formulation.

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Product Information

67481-1-PBS targets FURIN in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FURIN fusion protein Ag13313
• Full Name: furin (paired basic amino acid cleaving enzyme)
• Calculated Molecular Weight: 87 kDa
• Observed Molecular Weight: 90-110 kDa
• GenBank Accession Number: BC012181
• Gene Symbol: FURIN
• Gene ID (NCBI): 5045
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

FURIN, also named as FUR, PACE, PCSK3, SPC1, Kex2p, is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. Furin is synthesized as an inactive zymogen that may minimize the occurrence of premature enzymatic activity that would lead to alternative protein activation or degradation. Glycosylation and other post-translational modifications can make the pre-pro furin run at 100-110 kDa, the mature furin at 98-95 kDa and shed furin at 90 kDa.