Tested Applications
| Positive WB detected in | mouse liver tissue, COLO 320 cells |
| Positive IHC detected in | human testis tissue, human liver cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16420-1-AP targets FUCA1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9612 Product name: Recombinant human FUCA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 113-461 aa of BC017338 Sequence: EEWADLFQAAGAKYVVLTTKHHEGFTNWPSPVSWNWNSKDVGPHRDLVGELGTALRKRNIRYGLYHSLLEWFHPLYLLDKKNGFKTQHFVSAKTMPELYDLVNSYKPDLIWSDGEWECPDTYWNSTNFLSWLYNDSPVKDEVVVNDRWGQNCSCHHGGYYNCEDKFKPQSLPDHKWEMCTSIDKFSWGYRRDMALSDVTEESEIISELVQTVSLGGNYLLNIGPTKDGLIVPIFQERLLAVGKWLSINGEAIYASKPWRVQWEKNTTSVWYTSKGSAVYAIFLHWPENGVLNLESPITTSTTKITMLGIQGDLKWSTDPDKGLFISLPQLPPSAVPAEFAWTIKLTGVK Predict reactive species |
| Full Name | fucosidase, alpha-L- 1, tissue |
| Calculated Molecular Weight | 461 aa, 53 kDa |
| Observed Molecular Weight | 50-56 kDa |
| GenBank Accession Number | BC017338 |
| Gene Symbol | FUCA1 |
| Gene ID (NCBI) | 2517 |
| RRID | AB_2294111 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P04066 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FUCA1(a-l-Fucosidase) is a lysosomal glycosidase which is ubiquitous in eukaryotic cells. This enzyme is usually found as a soluble component of the lysosome and functions as an acid hydrolase in the degradation of numerous and diverse fucoglycoconjugates.The mammalian a-l-fucosidases are relatively large, multisubunit glycoproteins which exist in multiple molecular isoforms.This enzyme may have a role in sperm-egg interactions in the reproductive tract of the female rat. There is a precursor 54 kDa form of rat sperm fucosidase which is processed to a mature 52 kDa form of the enzyme(PMID:8836125). This protein from rat epididymis is a tetramer of 210-220 kDa, made up by two pairs of subunits of 47 and 60 kDa, and the one from human seminal plasma exists in different oligomeric forms, that is, as a dimer, as a tetramer, and as a hexamer made up of a single subunit of 56-57 kDa(PMID:16736526).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FUCA1 antibody 16420-1-AP | Download protocol |
| WB protocol for FUCA1 antibody 16420-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FUCA1 antibody (Cat# 16420-1-AP) has 11 total citations. Citations appear in 9 journals including Oncotarget, Cancer Cell International, Oncogenesis, Frontiers in Pharmacology, Frontiers in Aging Neuroscience, BMC Cancer, Gene, Cancer Genomics Proteomics, and Pathology International. Research fields are predominantly oncology—covering renal, colorectal, breast, thyroid, glioma, osteosarcoma, cervical, bladder, and mesothelioma cancers—with one study in neuroimmunology examining microglial activation. Applications include WB, IHC, and IF.
| Species | Application | Title |
|---|---|---|
Cancer Cell Int CTCF-activated FUCA1 functions as a tumor suppressor by promoting autophagy flux and serum α-L-fucosidase serves as a potential biomarker for prognosis in ccRCC | ||
Oncogenesis USP35 promotes cell proliferation and chemotherapeutic resistance through stabilizing FUCA1 in colorectal cancer | ||
Front Pharmacol Immune-related gene risk model establishment and role of key gene FUCA1 in malignant pleural mesothelioma | ||
Oncotarget Reduced expression of α-L-Fucosidase-1 (FUCA-1) predicts recurrence and shorter cancer specific survival in luminal B LN+ breast cancer patients. | ||
Front Aging Neurosci The temporal and stimuli-specific effects of LPS and IFNγ on microglial activation. | ||
