FSCN3 Polyclonal antibody

FSCN3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13536-1-AP

Synonyms

Testis fascin, Fascin-3, Fascin 3

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Validation Data Gallery

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  WB analysis of PC-3 using 13536-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13536-1-AP (FSCN3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 13536-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13536-1-AP (FSCN3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 13536-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13536-1-AP (FSCN3 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of PC-3 using 13536-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 13536-1-AP (FSCN3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of PC-3 using 13536-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using FSCN3 antibody (13536-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: PC-3 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13536-1-AP targets FSCN3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FSCN3 fusion protein Ag4459
• Full Name: fascin homolog 3, actin-bundling protein, testicular (Strongylocentrotus purpuratus)
• Calculated Molecular Weight: 498 aa, 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC035606
• Gene Symbol: FSCN3
• Gene ID (NCBI): 29999
• RRID: AB_2106517
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.