Tested Applications
| Positive WB detected in | HCT 116 cells, mouse heart tissue, HT-29 cells, mouse testis tissue, rat heart tissue, mouse kidney tissue, rat kidney tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21535-1-AP targets FOXO4 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, canine, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16169 Product name: Recombinant human FOXO4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 198-505 aa of BC106761 Sequence: MDSSSKLLRGRSKAPKKKPSVLPAPPEGATPTSPVGHFAKWSGSPCSRNREEADMWTTFRPRSSSNASSVSTRLSPLRPESEVLAEEIPASVSSYAGGVPPTLNEGLELLDGLNLTSSHSLLSRSGLSGFSLQHPGVTGPLHTYSSSLFSPAEGPLSAGEGCFSSSQALEALLTSDTPPPPADVLMTQVDPILSQAPTLLLLGGLPSSSKLATGVGLCPKPLEAPGPSSLVPTLSMIAPPPVMASAPIPKALGTPVLTPPTEAASQDRMPQDLDLDMYMENLECDMDNIISDLMDEGEGLDFNFEPDP Predict reactive species |
| Full Name | forkhead box O4 |
| Calculated Molecular Weight | 505 aa, 54 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC106761 |
| Gene Symbol | FOXO4 |
| Gene ID (NCBI) | 4303 |
| RRID | AB_10732960 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P98177 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FOXO4, also named as AFX, AFX1 and MLLT7, is a transcription factor which involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1.FOXO down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is involved in negative regulation of the cell cycle. A chromosomal aberration involving FOXO4 is found in acute leukemias. The antibody can recognize isoform 1 and isoform Zeta of FOXO4.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FOXO4 antibody 21535-1-AP | Download protocol |
| WB protocol for FOXO4 antibody 21535-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FOXO4 antibody (Cat# 21535-1-AP) has 27 citations across journals including Nature Communications, Molecular Cell, Journal of Clinical Investigation, Cell Death & Disease, Theranostics, FASEB Journal, and BMC Cancer. Research fields span oncology (glioblastoma, prostate cancer, NSCLC, colorectal cancer, leukemia), cardiovascular disease, cellular senescence, ferroptosis, viral infection, liver disease (NAFLD, HBV), fibrosis, placentation, and bone biology. Applications include WB, IHC, IF, and CoIP across human, mouse, rat, bovine, and canine species.
| Species | Application | Title |
|---|---|---|
Nat Commun Damaged brain accelerates bone healing by releasing small extracellular vesicles that target osteoprogenitors. | ||
Circ Res Suppression of Activated FOXO Transcription Factors in the Heart Prolongs Survival in a Mouse Model of Laminopathies. | ||
Redox Biol Notoginsenoside R1-loaded mesoporous silica nanoparticles targeting the site of injury through inflammatory cells improves heart repair after myocardial infarction. | ||
Theranostics An anti-FAP-scFv-functionalized exosome-carrying hydrogel delivers SKI mRNA to fibrotic nucleus pulposus cells to alleviate intervertebral disc degeneration by regulating FOXO3 | ||
J Clin Invest Hepatitis B virus X protein represses miRNA-148a to enhance tumorigenesis.
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Reviews
What customers say
The single review for 21535-1-AP is from a user who tested the antibody for immunohistochemistry on human skin tissue (lot 00014406). They rated it 5 out of 5 stars and commented that "it works pretty well for IHC." Overall, the feedback is positive, indicating satisfactory performance in IHC applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
zhiping (Verified Customer) (06-04-2021) | It works pretty well for IHC.
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