FOXJ2 Polyclonal antibody

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• KD/KO Validated

FOXJ2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28301-1-AP

Synonyms

FHX

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Validation Data Gallery

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  WB analysis using 28301-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28301-1-AP (FOXJ2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HuH-7 using 28301-1-AP

  WB result of FOXJ2 antibody (28301-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXJ2 transfected HuH-7 cells.

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Tested Applications

• Positive WB detected in: HuH-7 cells, U-87 MG cells, human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28301-1-AP targets FOXJ2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FOXJ2 fusion protein Ag27345
• Full Name: forkhead box J2
• Calculated Molecular Weight: 574 aa, 62 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC136305
• Gene Symbol: FOXJ2
• Gene ID (NCBI): 55810
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fork head box J2 (FOXJ2) is a transcription factor discovered in mammals and other vertebrates in 2000, which belongs to the Fork head box (Fox) transcription factor family, which shares a conserved DNA-binding domain, known as Fork Head. FOXJ2 participates in the regulation of cell proliferation, differentiation, and migration by targeting downstream genes through its DNA binding domain, and plays fundamental roles in embryonic development, tumorigenesis, and the progression of certain cancers. The expression patterns of FOXJ2 have been reported to be aberrant in a variety of cancers, including breast cancer, extra-hepatic cholangiocarcinoma, nasopharyngeal carcinoma, glioma, and non-small cell lung cancer (PMID: 28769576, PMID: 33725831, PMID: 35908066). This antibody has been validated for knockdown.