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  • KD/KO Validated

FMR1 Polyclonal antibody

FMR1 Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 13755-1-AP


FMR1, FMRP, fragile X mental retardation 1, FRAXA, POF, POF1, Protein FMR 1

Tested Applications

Positive WB detected inK-562 cells, mouse brain tissue, mouse thymus tissue, Jurkat cells, HeLa cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon tissue, human liver tissue, human colon cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, Hela cells
Positive FC detected inJurkat cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13755-1-AP targets FMR1 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FMR1 fusion protein Ag4697
Full Name fragile X mental retardation 1
Calculated Molecular Weight 632 aa, 71 kDa
Observed Molecular Weight 59-72 kDa
GenBank Accession NumberBC038998
Gene Symbol FMR1
Gene ID (NCBI) 2332
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm. FMR1 exists several isforms and the molecular weight of FMR1 is about 59-72 kDa.


Product Specific Protocols
WB protocol for FMR1 antibody 13755-1-APDownload protocol
IHC protocol for FMR1 antibody 13755-1-APDownload protocol
IF protocol for FMR1 antibody 13755-1-APDownload protocol
IP protocol for FMR1 antibody 13755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization.

Authors - David W Sanders

Cancer Cell Int

MiR-323a-3p acts as a tumor suppressor by suppressing FMR1 and predicts better esophageal squamous cell carcinoma outcome.

Authors - Yu Men

Biology (Basel)

In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.

Authors - Olga Gourdomichali

Cell Death Dis

FMR1 promotes the progression of colorectal cancer cell by stabilizing EGFR mRNA in an m6A-dependent manner

Authors - Yuhan Hu
  • KD Validated


Decreased DANCR contributes to high glucose-induced extracellular matrix accumulation in human renal mesangial cell via regulating the TGF-β/Smad signaling

Authors - Feng Liu

Dev Cell

Deregulation of ER-mitochondria contact formation and mitochondrial calcium homeostasis mediated by VDAC in fragile X syndrome

Authors - Ji Geng


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Parisa (Verified Customer) (09-26-2023)

We used for WB, nor high specificity as we could see different bands (they could be different isoforms of the protein). But it was sensitive and we could see the band pulled down in IP for our protein of interest.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Hek293

Marta (Verified Customer) (02-24-2020)

not specific enough in WB. Not tested in IPworst than the antibody that we have available in the antibody. Need a long exposure with a high sensible ECL.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Brain mouse and rat
FMR1 Antibody Western Blot, validation (1/1000 dilution) in Brain mouse and rat (Cat no:13755-1-AP)