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FMR1 Polyclonal antibody
FMR1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Conjugate
Unconjugated
Cat no : 13755-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | K-562 cells, mouse brain tissue, mouse thymus tissue, Jurkat cells, HeLa cells, HEK-293 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human colon tissue, human liver tissue, human colon cancer tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells, Hela cells |
Positive FC (Intra) detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 7 publications below |
IHC | See 2 publications below |
Product Information
13755-1-AP targets FMR1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | FMR1 fusion protein Ag4697 |
Full Name | fragile X mental retardation 1 |
Calculated Molecular Weight | 632 aa, 71 kDa |
Observed Molecular Weight | 59-72 kDa |
GenBank Accession Number | BC038998 |
Gene Symbol | FMR1 |
Gene ID (NCBI) | 2332 |
RRID | AB_2262872 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm. FMR1 exists several isforms and the molecular weight of FMR1 is about 59-72 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for FMR1 antibody 13755-1-AP | Download protocol |
IHC protocol for FMR1 antibody 13755-1-AP | Download protocol |
IF protocol for FMR1 antibody 13755-1-AP | Download protocol |
IP protocol for FMR1 antibody 13755-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization. | ||
Nat Commun Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis | ||
Cell Rep hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility | ||
Cancer Cell Int MiR-323a-3p acts as a tumor suppressor by suppressing FMR1 and predicts better esophageal squamous cell carcinoma outcome. | ||
Biology (Basel) In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome. | ||
Cell Death Dis FMR1 promotes the progression of colorectal cancer cell by stabilizing EGFR mRNA in an m6A-dependent manner
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Parisa (Verified Customer) (09-26-2023) | We used for WB, nor high specificity as we could see different bands (they could be different isoforms of the protein). But it was sensitive and we could see the band pulled down in IP for our protein of interest.
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FH Marta (Verified Customer) (02-24-2020) | not specific enough in WB. Not tested in IPworst than the antibody that we have available in the antibody. Need a long exposure with a high sensible ECL.
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