• Featured Product
  • KD/KO Validated

Flotillin 1 Polyclonal antibody

Flotillin 1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 15571-1-AP

Synonyms

FLOT1, Flotillin-1



Tested Applications

Positive WB detected inmouse brain tissue, U-251 cells, mouse heart tissue, rat brain tissue, rat heart tissue, U-87 MG cells
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15571-1-AP targets Flotillin 1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Flotillin 1 fusion protein Ag7902
Full Name flotillin 1
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC001146
Gene Symbol Flotillin 1
Gene ID (NCBI) 10211
RRIDAB_2106746
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins commonly used as lipid raft markers. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.

Protocols

Product Specific Protocols
WB protocol for Flotillin 1 antibody 15571-1-APDownload protocol
IP protocol for Flotillin 1 antibody 15571-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Extracell Vesicles

Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.

Authors - Xiaohan Gao
humanWB

Brain

Extracellular vesicle biomarkers for complement dysfunction in schizophrenia

Authors - Ting Xue
human,mouseWB

Theranostics

Microenvironment-induced CREPT expression by cancer-derived small extracellular vesicles primes field cancerization

Authors - Yuting Lin
mouseWB

Cell Res

Translocation of mixed lineage kinase domain-like protein to plasma membrane leads to necrotic cell death.

Authors - Xin Chen
human,mouseWB,IHC

Int J Biol Sci

FLOT1 promotes gastric cancer progression and metastasis through BCAR1/ERK signaling

Authors - Ran Wang
  • KD Validated
ratWB

Cell Death Dis

Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats

Authors - Yajie Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Guorong (Verified Customer) (03-22-2022)

Good performance with a specific band of expected size and 2 bigger and weaker bands

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2500
  • Cell Tissue Type: ARPE-19
Flotillin 1 Antibody Western Blot validation (1:2500 dilution) in ARPE-19 (Cat no:15571-1-AP)