Flotillin 1 Polyclonal antibody

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  WB analysis using 15571-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15571-1-AP (Flotillin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15571-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15571-1-AP (Flotillin 1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15571-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15571-1-AP (Flotillin 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 15571-1-AP

  IP result of anti-Flotillin 1 (IP:15571-1-AP, 4ug; Detection:15571-1-AP 1:500) with mouse brain tissue lysate 4400ug.

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  IHC staining of human stomach using 15571-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 15571-1-AP (Flotillin 1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15571-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Flotillin 1 antibody (15571-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

Flotillin 1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15571-1-AP

Publications(55)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

FLOT1, Flotillin-1

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Tested Applications

• Positive WB detected in: mouse brain tissue, U-251 cells, mouse heart tissue, rat brain tissue, rat heart tissue, U-87 MG cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 51 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below

Product Information

15571-1-AP targets Flotillin 1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7902

  Product name: Recombinant human Flotillin 1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 80-427 aa of BC001146

  Sequence: MLAAACQMFLGKTEAEIAHIALETLEGHQRAIMAHMTVEEIYKDRQKFSEQVFKVASSDLVNMGISVVSYTLKDIHDDQDYLHSLGKARTAQVQKDARIGEAEAKRDAGIREAKAKQEKVSAQYLSEIEMAKAQRDYELKKAAYDIEVNTRRAQADLAYQLQVAKTKQQIEEQRVQVQVVERAQQVAVQEQEIARREKELEARVRKPAEAERYKLERLAEAEKSQLIMQAEAEAASVRMRGEAEAFAIGARARAEAEQMAKKAEAFQLYQEAAQLDMLLEKLPQVAEEISGPLTSANKITLVSSGSGTMGAAKVTGEVLDILTRLPESVERLTGVSISQVNHKPLRTA

• Full Name: flotillin 1
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC001146
• Gene Symbol: Flotillin 1
• Gene ID (NCBI): 10211
• RRID: AB_2106746
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75955
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins commonly used as lipid raft markers. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Extracell Vesicles; Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.; Authors - Xiaohan Gao
human	WB	Brain; Extracellular vesicle biomarkers for complement dysfunction in schizophrenia; Authors - Ting Xue
mouse,human	WB	Theranostics; Microenvironment-induced CREPT expression by cancer-derived small extracellular vesicles primes field cancerization; Authors - Yuting Lin
mouse	WB	Cell Res; Translocation of mixed lineage kinase domain-like protein to plasma membrane leads to necrotic cell death.; Authors - Xin Chen
mouse,human	WB,IHC	Int J Biol Sci; FLOT1 promotes gastric cancer progression and metastasis through BCAR1/ERK signaling; Authors - Ran Wang ; KD Validated
rat	WB	Cell Death Dis; Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats; Authors - Yajie Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Guorong (Verified Customer) (03-22-2022)

Good performance with a specific band of expected size and 2 bigger and weaker bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: ARPE-19

Flotillin 1 Antibody Western Blot validation (1:2500 dilution) in ARPE-19 (Cat no:15571-1-AP)