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Flotillin 1 Polyclonal antibody
Flotillin 1 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 15571-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, U-251 cells, mouse heart tissue, rat brain tissue, rat heart tissue, U-87 MG cells |
Positive IP detected in | mouse brain tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 44 publications below |
IHC | See 3 publications below |
IF | See 2 publications below |
IP | See 3 publications below |
CoIP | See 2 publications below |
Product Information
15571-1-AP targets Flotillin 1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Flotillin 1 fusion protein Ag7902 |
Full Name | flotillin 1 |
Calculated Molecular Weight | 47 kDa |
Observed Molecular Weight | 47 kDa |
GenBank Accession Number | BC001146 |
Gene Symbol | Flotillin 1 |
Gene ID (NCBI) | 10211 |
RRID | AB_2106746 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins commonly used as lipid raft markers. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.
Protocols
Product Specific Protocols | |
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WB protocol for Flotillin 1 antibody 15571-1-AP | Download protocol |
IP protocol for Flotillin 1 antibody 15571-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Extracell Vesicles Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway. | ||
Theranostics Microenvironment-induced CREPT expression by cancer-derived small extracellular vesicles primes field cancerization | ||
Cell Res Translocation of mixed lineage kinase domain-like protein to plasma membrane leads to necrotic cell death. | ||
Int J Biol Sci FLOT1 promotes gastric cancer progression and metastasis through BCAR1/ERK signaling
| ||
Cell Death Dis Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Guorong (Verified Customer) (03-22-2022) | Good performance with a specific band of expected size and 2 bigger and weaker bands
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