Filamin B Polyclonal antibody

Filamin B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 20685-1-AP

Synonyms

FLNB, FilaminB, Filamin-3, Beta-filamin, Actin-binding-like protein

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Validation Data Gallery

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  WB analysis of HeLa using 20685-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20685-1-AP (FLNB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U2OS using 20685-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 20685-1-AP (FLNB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 20685-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20685-1-AP (FLNB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 20685-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20685-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 20685-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 20685-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon using 20685-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 20685-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 20685-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 20685-1-AP

  Immunofluorescent analysis of HepG2 cells, using FLNB antibody 20685-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells, U2OS cells, NIH/3T3 cells
• Positive IHC detected in: human liver cancer tissue, human normal colon, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

20685-1-AP targets Filamin B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: filamin B, beta (actin binding protein 278)
• Calculated Molecular Weight: 278 kDa
• Observed Molecular Weight: 250-278 kDa
• GenBank Accession Number: NM_001457
• Gene Symbol: FLNB
• Gene ID (NCBI): 2317
• RRID: AB_10863658
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FLNB, also named as FLN1L, FLN3, TABP, TAP and Fh1, belongs to the filamin family. FLNB connects cell membrane constituents to the actin cytoskeleton. It may promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. It is an anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA, FLNB may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. The antibody is specific to FLNB. It can recognize isoform1,2,3,6,7 of FLNB. It has no cross reaction to FLNA and FLNC.

Publications

Species	Application	Title
human	WB	Cell Death Dis; NudCL2 regulates cell migration by stabilizing both myosin-9 and LIS1 with Hsp90.; Authors - Wenwen Chen
mouse	IHC	J Genet Genomics; Rare loss-of-function variants in FLNB cause non-syndromic orofacial clefts; Authors - Wenbin Huang
human,mouse	IHC,IF	Am J Cancer Res; FLNB overexpression promotes tumor progression and associates with immune suppression, evasion and stemness in pancreatic cancer; Authors - Guoyong Fan