AAGAB Polyclonal antibody

AAGAB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27319-1-AP

Synonyms

AAGAB, FLJ11506

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Validation Data Gallery

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  WB analysis of K-562 using 27319-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 27319-1-AP (AAGAB antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 27319-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 27319-1-AP (AAGAB antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse thymus using 27319-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 27319-1-AP (AAGAB antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse thymus using 27319-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 27319-1-AP (AAGAB antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat thymus using 27319-1-AP

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 27319-1-AP (AAGAB antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat thymus using 27319-1-AP

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 27319-1-AP (AAGAB antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: K-562 cells, mouse thymus tissue
• Positive IHC detected in: mouse thymus tissue, rat thymus tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 27319-1-AP is AAGAB Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AAGAB fusion protein Ag26340
• Full Name: alpha- and gamma-adaptin-binding protein p34
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC058886
• Gene Symbol: AAGAB
• Gene ID (NCBI): 79719
• RRID: AB_2880841
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Recent study indicate that mutations in AAGAB can cause punctate palmoplantar keratoderma type I (PPKP1; OMIM#148600), also called keratosis punctata palmoplantaris Buschke-Fischer-Brauer. The predicted MW of this protein is 35 kDa. Catalog#27319-1-AP recognises 37-40 kDa band may due to phosphorylation.