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FH Polyclonal antibody

FH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11375-1-AP

Synonyms

HsFH, Fumarase, EC:4.2.1.2



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, human placenta tissue, Hela cells, Jurkat cells, NIH/3T3 cells, C6 cells, mouse liver tissue, rat liver tissue, L02 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman placenta tissue, human normal colon, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11375-1-AP targets FH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, caenorhabditis elegans
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FH fusion protein Ag1934
Full Name fumarate hydratase
Calculated Molecular Weight 510 aa, 55 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC003108
Gene Symbol FH
Gene ID (NCBI) 2271
RRIDAB_763171
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fumarate hydratase, or fumarase, is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. It belongs to the class-II fumarase/aspartase family. This protein has 2 isoforms produced by alternative initiation.

Protocols

Product Specific Protocols
WB protocol for FH antibody 11375-1-APDownload protocol
IHC protocol for FH antibody 11375-1-APDownload protocol
IF protocol for FH antibody 11375-1-APDownload protocol
IP protocol for FH antibody 11375-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.

Authors - Xiaoyu Ma
mouseWB

Aging Dis

Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging

Authors - Minghao Yuan
humanWB

Theranostics

EBV(LMP1)-induced metabolic reprogramming inhibits necroptosis through the hypermethylation of the RIP3 promoter.

Authors - Feng Shi
humanWB

Oncotarget

CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.

Authors - Zhichao Gu
humanWB

Invest Ophthalmol Vis Sci

Proteomics of uveal melanomas suggests HSP-27 as a possible surrogate marker of chromosome 3 loss.

Authors - Coupland Sarah E SE
Caenorhabditis elegansWB

Int J Radiat Biol

Coupling of oxidative stress responses to tricarboxylic acid cycle and prostaglandin E2 alterations in Caenorhabditis elegans under extremely low-frequency electromagnetic field.

Authors - Yongyan Sun