FH Polyclonal antibody

FH Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 11375-1-AP


FH, Fumarase, fumarate hydratase, HLRCC, LRCC, MCL, MCUL1

Tested Applications

Positive WB detected inHEK-293 cells, human placenta tissue, HepG2 cells, L02 cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue, Hela cells, Jurkat cells, C6 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman placenta tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11375-1-AP targets FH in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Caenorhabditis elegans
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FH fusion protein Ag1934
Full Name fumarate hydratase
Calculated Molecular Weight 510 aa, 55 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC003108
Gene Symbol FH
Gene ID (NCBI) 2271
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fumarate hydratase, or fumarase, is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. It belongs to the class-II fumarase/aspartase family. This protein has 2 isoforms produced by alternative initiation.


Product Specific Protocols
WB protocol for FH antibody 11375-1-APDownload protocol
IHC protocol for FH antibody 11375-1-APDownload protocol
IF protocol for FH antibody 11375-1-APDownload protocol
IP protocol for FH antibody 11375-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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