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FH Polyclonal antibody
FH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 11375-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HepG2 cells, human placenta tissue, Hela cells, Jurkat cells, NIH/3T3 cells, C6 cells, mouse liver tissue, rat liver tissue, L02 cells |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | human placenta tissue, human normal colon, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 11 publications below |
IHC | See 4 publications below |
IF | See 1 publications below |
Product Information
11375-1-AP targets FH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, caenorhabditis elegans |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | FH fusion protein Ag1934 |
Full Name | fumarate hydratase |
Calculated Molecular Weight | 510 aa, 55 kDa |
Observed Molecular Weight | 45-50 kDa |
GenBank Accession Number | BC003108 |
Gene Symbol | FH |
Gene ID (NCBI) | 2271 |
RRID | AB_763171 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fumarate hydratase, or fumarase, is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. It belongs to the class-II fumarase/aspartase family. This protein has 2 isoforms produced by alternative initiation.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for FH antibody 11375-1-AP | Download protocol |
IHC protocol for FH antibody 11375-1-AP | Download protocol |
IF protocol for FH antibody 11375-1-AP | Download protocol |
IP protocol for FH antibody 11375-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1. | ||
Aging Dis Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging | ||
Theranostics EBV(LMP1)-induced metabolic reprogramming inhibits necroptosis through the hypermethylation of the RIP3 promoter. | ||
Invest Ophthalmol Vis Sci Proteomics of uveal melanomas suggests HSP-27 as a possible surrogate marker of chromosome 3 loss. | ||
Int J Radiat Biol Coupling of oxidative stress responses to tricarboxylic acid cycle and prostaglandin E2 alterations in Caenorhabditis elegans under extremely low-frequency electromagnetic field. |