Validation Data Gallery
|Positive WB detected in||HEK-293 cells, human placenta tissue, HepG2 cells, L02 cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue, Hela cells, Jurkat cells, C6 cells|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human placenta tissue, human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:2000-1:16000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11375-1-AP targets FH in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, Caenorhabditis elegans|
|Host / Isotype||Rabbit / IgG|
|Immunogen||FH fusion protein Ag1934|
|Full Name||fumarate hydratase|
|Calculated molecular weight||510 aa, 55 kDa|
|Observed molecular weight||45-50 kDa|
|GenBank accession number||BC003108|
|Gene ID (NCBI)||2271|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Fumarate hydratase, or fumarase, is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. It belongs to the class-II fumarase/aspartase family. This protein has 2 isoforms produced by alternative initiation.
|Product Specific Protocols|
|WB protocol for FH antibody 11375-1-AP||Download protocol|
|IHC protocol for FH antibody 11375-1-AP||Download protocol|
|IF protocol for FH antibody 11375-1-AP||Download protocol|
|IP protocol for FH antibody 11375-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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