FGFBP1 Polyclonal antibody

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  IHC staining of human skin cancer using 25006-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 25006-1-AP (FGFBP1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin cancer using 25006-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 25006-1-AP (FGFBP1 antibody) at dilution of 1:200 (under 40x lens).

FGFBP1 Polyclonal Antibody for IHC, ELISA

Cat No. 25006-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Synonyms

17 kDa HBGF binding protein, 17 kDa HBGF-binding protein, 17 kDa heparin-binding growth factor-binding protein, FGF binding protein 1, FGF BP

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Tested Applications

• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

25006-1-AP targets FGFBP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6357

  Product name: Recombinant human FGFBP1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-234 aa of BC003628

  Sequence: MKICSLTLLSFLLLAAQVLLVEGKKKVKNGLHSKVVSEQKDTLGNTQIKQKSRPGNKGKFVTKDQANCRWAATEQEEGISLKVECTQLDHEFSCVFAGNPTSCLKLKDERVYWKQVARNLRSQKDICRYSKTAVKTRVCRKDFPESSLKLVSSTLFGNTKPRKEKTEMSPREHIKGKETTPSSLAVTQTMATKAPECVEDPDMANQRKTALEFCGETWSSLCTFFLSIVQDTSC

• Full Name: fibroblast growth factor binding protein 1
• Calculated Molecular Weight: 26 kDa
• GenBank Accession Number: BC003628
• Gene Symbol: FGFBP1
• Gene ID (NCBI): 9982
• RRID: AB_2879843
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14512
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FGFBP1, also named as HBP17, is prominent for its role as the chaperone for fibroblast growth factor-2 (FGF-2), which plays a crucial role in angiogenesis as well as promoting tumor growth. HBp17/FGFBP-1 has been proposed as a candidate biomarker for a number of cancers since it is frequently found to be elevated in many cancer types including in the tissue and cell lines of oral squamous cell carcinomas (OSCC).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Mol Cell; A transient transcriptional activation governs unpolarized-to-polarized morphogenesis during embryo implantation; Authors - Xuehui Lyu
human	WB,IHC	Front Immunol; FGFBP1 as a potential biomarker predicting bacillus Calmette-Guérin response in bladder cancer; Authors - Fei Li
mouse	WB,IHC	Am J Cancer Res; Type-2 11β-hydroxysteroid dehydrogenase promotes the metastasis of colorectal cancer via the Fgfbp1-AKT pathway.; Authors - Jin Chen ; KD Validated
human	WB,IHC	Transl Oncol; Identification of metastasis-associated exoDEPs in colorectal cancer using label-free proteomics.; Authors - Xinlu Liu
mouse,human	WB,IHC	Front Pharmacol; B4 suppresses lymphoma progression by inhibiting fibroblast growth factor binding protein 1 through intrinsic apoptosis; Authors - Krishnapriya M Varier
human	WB	Pharm Biol; Study on the biological mechanism of urolithin a on nasopharyngeal carcinoma in vitro; Authors - Yang Yang