FGF-21 Polyclonal antibody

FGF-21 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 26272-1-AP

Synonyms

FGF21, UNQ3115/PRO10196, Fibroblast growth factor 21, FGF 21

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Validation Data Gallery

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  IHC staining of human lung cancer using 26272-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26272-1-AP (FGF21 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 26272-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26272-1-AP (FGF21 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 26272-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26272-1-AP (FGF21 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 26272-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26272-1-AP (FGF21 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse liver using 26272-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FGF21 antibody (26272-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse liver using 26272-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FGF21 antibody (26272-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human lung cancer tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse liver tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

26272-1-AP targets FGF-21 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FGF-21 fusion protein Ag24064
• Full Name: fibroblast growth factor 21
• Calculated Molecular Weight: 22 kDa
• GenBank Accession Number: BC018404
• Gene Symbol: FGF21
• Gene ID (NCBI): 26291
• RRID: AB_2880455
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibroblast growth factor 21 (FGF21), a member of the FGF superfamily, has emerged as an important metabolic regulator. FGF21 is produced in the liver, adipocytes and skeletal muscle and regulates glucose, energy and lipid metabolism. FGF21 is a stress-responsive hepatokine which is induced in the liver as a response to injury, regeneration, hepatocarcinogenesis and during kidney dysfunction. FGF21 is primarily metabolized in the kidney and renal secretion is the major route of FGF21 elimination. Its plasma levels increase according to the decline in renal function in patients with chronic and acute renal dysfunction.

Publications

Species	Application	Title
mouse		Nat Commun; The mitochondrial fusion protein OPA1 is dispensable in the liver and its absence induces mitohormesis to protect liver from drug-induced injury; Authors - Hakjoo Lee
human		Biomed Pharmacother; Dihydroartemisinin inhibits ER stress-mediated mitochondrial pathway to attenuate hepatocyte lipoapoptosis via blocking the activation of the PI3K/Akt pathway.; Authors - Xingran Chen
rat	IF	Cell Death Dis; FGF21 augments autophagy in random-pattern skin flaps via AMPK signaling pathways and improves tissue survival.; Authors - Kailiang Zhou
mouse		Sci Rep; Brown adipose tissue-derived FGF21 mediates the cardioprotection of dexmedetomidine in myocardial ischemia/reperfusion injury; Authors - Yi Ding