FEV Polyclonal antibody

FEV Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25058-1-AP

Synonyms

PET 1, PC12 ETS factor 1, PC12 ETS domain-containing transcription factor 1, HSRNAFEV, Fifth Ewing variant protein

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Validation Data Gallery

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  WB analysis of fetal human brain using 25058-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 25058-1-AP (FEV Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25058-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25058-1-AP (FEV antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: fetal human brain tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

25058-1-AP targets FEV in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FEV fusion protein Ag20647
• Full Name: FEV (ETS oncogene family)
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC023511
• Gene Symbol: FEV
• Gene ID (NCBI): 54738
• RRID: AB_2879877
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FEV belongs to the ETS transcription factor family. ETS family members have a highly conserved 85-amino acid ETS domain that binds purine-rich DNA sequences. The alanine-rich C-terminus of this gene indicates that it may act as a transcription repressor. This gene is exclusively expressed in neurons of the central serotonin (5-HT) system, a system implicated in the pathogeny of such psychiatric diseases as depression, anxiety, and eating disorders. In some types of Ewing tumors, this gene is fused to the Ewing sarcoma (EWS) gene following chromosome translocations.

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda