FBXO41 Polyclonal antibody

FBXO41 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 24519-1-AP

Synonyms

F box only protein 41, F box protein 41, Fbx41, FBXO41, KIAA1940

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Validation Data Gallery

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  WB analysis using 24519-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24519-1-AP (FBXO41 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 24519-1-AP

  IP result of anti-FBXO41 (IP:24519-1-AP, 4ug; Detection:24519-1-AP 1:1000) with mouse brain tissue lysate 6000ug.

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  IHC staining of mouse brain using 24519-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24519-1-AP (FBXO41 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 24519-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24519-1-AP (FBXO41 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 24519-1-AP is FBXO41 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FBXO41 fusion protein Ag21595
• Full Name: F-box protein 41
• Calculated Molecular Weight: 875 aa, 94 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC112202
• Gene Symbol: FBXO41
• Gene ID (NCBI): 150726
• RRID: AB_2879586
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBXO41 also named as F box only protein 41 is a 875 amino-acid protein, which directly interact with SKP1 and CUL1. FBXO41 is identified as a Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex, which as a regulator mediates some protein ubiquitination degradation. FBXO41 consists of F-box domain and has zinc ion binding activity. FBXO41 may have an association with Parkinson's disease.

Publications

Species	Application	Title
	WB	Sci Adv; Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.; Authors - Odetta Antico
rat	WB	iScience; Neuronal F-Box protein FBXO41 regulates synaptic transmission and hippocampal network maturation.; Authors - Ana R A A Quadros