Fibulin 5 Polyclonal antibody

• Featured Product
• KD/KO Validated

Fibulin 5 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 12188-1-AP

Synonyms

ARMD3, DANCE, EVEC, FBLN5, FIBL 5, fibulin 5, UP50, Urine p50 protein

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Validation Data Gallery

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  WB analysis using 12188-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12188-1-AP (Fibulin 5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12188-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12188-1-AP (Fibulin 5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 12188-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12188-1-AP (Fibulin 5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 12188-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12188-1-AP (Fibulin 5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 12188-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 12188-1-AP (Fibulin 5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12188-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12188-1-AP (Fibulin 5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 12188-1-AP

  IP result of anti-Fibulin 5 (IP:12188-1-AP, 3ug; Detection:12188-1-AP 1:300) with mouse heart tissue lysate 8000ug.

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  IHC staining of mouse lung using 12188-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 12188-1-AP (Fibulin 5 antibody at dilution of 1:200.

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  IHC staining of mouse lung using 12188-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 12188-1-AP (Fibulin 5 antibody at dilution of 1:200.

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  IHC staining of human heart using 12188-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12188-1-AP (Fibulin 5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 12188-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12188-1-AP (Fibulin 5 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 12188-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 12188-1-AP (Fibulin 5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 12188-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 12188-1-AP (Fibulin 5 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat brain tissue, mouse placenta tissue, mouse skeletal muscle tissue, mouse kidney tissue, rat heart tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: mouse lung tissue, human heart tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

12188-1-AP targets Fibulin 5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Fibulin 5 fusion protein Ag2859
• Full Name: fibulin 5
• Calculated Molecular Weight: 448 aa, 50 kDa
• Observed Molecular Weight: 55 kDa, 65 kDa
• GenBank Accession Number: BC022280
• Gene Symbol: Fibulin 5
• Gene ID (NCBI): 10516
• RRID: AB_2105939
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibulin-5, also known as EVEC and DANCE, is a extracellular matrix glycoprotein, secreted by various cell types such as vascular smooth muscle cells, fibroblasts and endothelial cells. Structurally, Fibulin-5 contains an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies. This antibody got 50 kDa or 66 kDa band in western blotting, the 66 kDa corresponding to fully glycosylated protein.

Publications

Species	Application	Title
mouse	WB	Arterioscler Thromb Vasc Biol; Increased aortic stiffness and attenuated lysyl oxidase activity in obesity.; Authors - Chen Ju-Yi JY
human	WB	Biochem J; Fibulin-5 binds urokinase-type plasminogen activator and mediates urokinase-stimulated β1-integrin-dependent cell migration.; Authors - Kapustin Alexander A
mouse	WB, IHC	Cell Tissue Res; Aberrant elastin remodeling in the lungs of O2-exposed newborn mice; primarily results from perturbed interaction between integrins and elastin.; Authors - Wenli Han
mouse	WB	Am J Physiol Cell Physiol; Caveolin-1 stabilizes ATP7A, Cu transporter for Extracellular SOD, in Vascular Tissue to Maintain Endothelial Function.; Authors - Varadarajan Sudhahar
human	WB	Clin Exp Pharmacol Physiol; Limb remote ischemic postconditioning-induced elevation of fibulin-5 confers neuroprotection to rats with cerebral ischemia/reperfusion injury: activation of the AKT pathway.; Authors - Wei Zhang ; KD Validated
human	IF	JID Innov; Fibulin-4 Accelerates Amyloid Formation by Binding with a Keratin 5 Peptide Fragment.; Authors - Fumihiko Katagiri