Tested Applications
| Positive WB detected in | mouse heart tissue, rat brain tissue, mouse placenta tissue, mouse skeletal muscle tissue, mouse kidney tissue, rat heart tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | mouse lung tissue, human heart tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12188-1-AP targets Fibulin 5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2859 Product name: Recombinant human FBLN5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 186-448 aa of BC022280 Sequence: GSYSCTCNPGFTLNEDGRSCQDVNECATENPCVQTCVNTYGSFICRCDPGYELEEDGVHCSDMDECSFSEFLCQHECVNQPGTYFCSCPPGYILLDDNRSCQDINECEHRNHTCNLQQTCYNLQGGFKCIDPIRCEEPYLRISDNRCMCPAENPGCRDQPFTILYRDMDVVSGRSVPADIFQMQATTRYPGAYYIFQIKSGNEGREFYMRQTGPISATLVMTRPIKGPREIQLDLEMITVNTVINFRGSSVIRLRIYVSQYPF Predict reactive species |
| Full Name | fibulin 5 |
| Calculated Molecular Weight | 448 aa, 50 kDa |
| Observed Molecular Weight | 55 kDa, 65 kDa |
| GenBank Accession Number | BC022280 |
| Gene Symbol | Fibulin 5 |
| Gene ID (NCBI) | 10516 |
| RRID | AB_2105939 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBX5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fibulin-5, also known as EVEC and DANCE, is a extracellular matrix glycoprotein, secreted by various cell types such as vascular smooth muscle cells, fibroblasts and endothelial cells. Structurally, Fibulin-5 contains an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies. This antibody got 50 kDa or 66 kDa band in western blotting, the 66 kDa corresponding to fully glycosylated protein.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Fibulin 5 antibody 12188-1-AP | Download protocol |
| IP protocol for Fibulin 5 antibody 12188-1-AP | Download protocol |
| WB protocol for Fibulin 5 antibody 12188-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-Fibulin-5 antibody (Cat# 12188-1-AP) has 13 citations spanning journals including Cell Reports, JHEP Reports, J Biomedical Science, Arteriosclerosis, Thrombosis and Vascular Biology, iScience, Journal of Investigative Dermatology, Molecular Carcinogenesis, Biochemical Journal, American Journal of Physiology, Cell and Tissue Research, Clinical and Experimental Pharmacology and Physiology, and Journal of Interferon & Cytokine Research. Research fields cover cardiovascular disease, obesity, cancer radioresistance, elastic fiber biology, β-cell proliferation, neuroprotection, and pulmonary development.
| Species | Application | Title |
|---|---|---|
J Biomed Sci PPARγ activation improves the microenvironment of perivascular adipose tissue and attenuates aortic stiffening in obesity. | ||
Arterioscler Thromb Vasc Biol Increased aortic stiffness and attenuated lysyl oxidase activity in obesity. | ||
JHEP Rep Biliary atresia susceptibility gene EFEMP1 regulates extrahepatic bile duct elastic fiber formation and mechanics | ||
Cell Rep Cancer-associated fibroblast-derived fibulin-5 promotes radioresistance in non-small-cell lung cancer
| ||
J Invest Dermatol Identification of fibulin-5 as a granzyme B substrate involved in UVB-induced elastic fiber disruption. | ||
Am J Physiol Cell Physiol Caveolin-1 stabilizes ATP7A, Cu transporter for Extracellular SOD, in Vascular Tissue to Maintain Endothelial Function. |
