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  • KD/KO Validated

Fibulin 5 Polyclonal antibody

Fibulin 5 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 12188-1-AP


ARMD3, DANCE, EVEC, FBLN5, FIBL 5, fibulin 5, UP50, Urine p50 protein

Tested Applications

Positive WB detected inmouse heart tissue, rat brain tissue, mouse placenta tissue, mouse skeletal muscle tissue, mouse kidney tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inmouse lung tissue, human heart tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12188-1-AP targets Fibulin 5 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Fibulin 5 fusion protein Ag2859
Full Name fibulin 5
Calculated Molecular Weight 448 aa, 50 kDa
Observed Molecular Weight55 kDa, 65 kDa
GenBank Accession NumberBC022280
Gene Symbol FBLN5
Gene ID (NCBI) 10516
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibulin-5, also known as EVEC and DANCE, is a extracellular matrix glycoprotein, secreted by various cell types such as vascular smooth muscle cells, fibroblasts and endothelial cells. Structurally, Fibulin-5 contains an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies. This antibody got 50 kDa or 66 kDa band in western blotting, the 66 kDa corresponding to fully glycosylated protein.


Product Specific Protocols
WB protocol for Fibulin 5 antibody 12188-1-APDownload protocol
IHC protocol for Fibulin 5 antibody 12188-1-APDownload protocol
IP protocol for Fibulin 5 antibody 12188-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Arterioscler Thromb Vasc Biol

Increased aortic stiffness and attenuated lysyl oxidase activity in obesity.

Authors - Chen Ju-Yi JY

Biochem J

Fibulin-5 binds urokinase-type plasminogen activator and mediates urokinase-stimulated β1-integrin-dependent cell migration.

Authors - Kapustin Alexander A
mouseWB, IHC

Cell Tissue Res

Aberrant elastin remodeling in the lungs of O2-exposed newborn mice; primarily results from perturbed interaction between integrins and elastin.

Authors - Wenli Han

Am J Physiol Cell Physiol

Caveolin-1 stabilizes ATP7A, Cu transporter for Extracellular SOD, in Vascular Tissue to Maintain Endothelial Function.

Authors - Varadarajan Sudhahar

Clin Exp Pharmacol Physiol

Limb remote ischemic postconditioning-induced elevation of fibulin-5 confers neuroprotection to rats with cerebral ischemia/reperfusion injury: activation of the AKT pathway.

Authors - Wei Zhang
  • KD Validated

JID Innov

Fibulin-4 Accelerates Amyloid Formation by Binding with a Keratin 5 Peptide Fragment.

Authors - Fumihiko Katagiri