FAM92B Polyclonal antibody

FAM92B Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 26413-1-AP

Synonyms

FAM92B, Protein FAM92B

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Validation Data Gallery

• 

  WB analysis of HeLa using 26413-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26413-1-AP (FAM92B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 26413-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 26413-1-AP (FAM92B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 26413-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 26413-1-AP (FAM92B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse lung using 26413-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using FAM92B antibody (26413-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse lung using 26413-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using FAM92B antibody (26413-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, COLO 320 cells, mouse small intestine tissue
• Positive IF-P detected in: mouse lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26413-1-AP targets FAM92B in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM92B fusion protein Ag24802
• Full Name: family with sequence similarity 92, member B
• Calculated Molecular Weight: 304 aa, 35 kDa
• Observed Molecular Weight: 32-35 kDa
• GenBank Accession Number: BC111944
• Gene Symbol: FAM92B
• Gene ID (NCBI): 339145
• RRID: AB_2880506
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Eunice (Verified Customer) (10-24-2022)

This was tested on mouse tracheal epithelial cells fixed in 4% PFA overnight and on MEFs fixed for 10 min in ice-cold methanol acetone. Works well.

• Applications: Immunofluorescence, Cell culture
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Tracheal epithelial cells, mouse embryonic fibroblasts (MEFs)